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The neurovirulence and neuroinvasiveness of chimeric tick-borne encephalitis/dengue virus can be attenuated by introducing defined mutations into the envelope and NS5 protein genes and the 3' non-coding region of the genome.
Engel AR, Rumyantsev AA, Maximova OA, Speicher JM, Heiss B, Murphy BR, Pletnev AG. Engel AR, et al. Virology. 2010 Sep 15;405(1):243-52. doi: 10.1016/j.virol.2010.06.014. Epub 2010 Jul 1. Virology. 2010. PMID: 20594569 Free PMC article.