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Programmable RNA detection with CRISPR-Cas12a.
Rananaware SR, Vesco EK, Shoemaker GM, Anekar SS, Sandoval LSW, Meister KS, Macaluso NC, Nguyen LT, Jain PK. Rananaware SR, et al. Nat Commun. 2023 Sep 5;14(1):5409. doi: 10.1038/s41467-023-41006-1. Nat Commun. 2023. PMID: 37669948 Free PMC article.
DNA-guided CRISPR/Cas12 for RNA targeting.
Orosco C, Rananaware SR, Huang B, Hanna MP, Ahmadimashhadi MR, Lewis JG, Baugh MP, Bodin AP, Flannery SJ, Lange IH, Fang ZR, Karalkar VN, Meister KS, Jain PK. Orosco C, et al. medRxiv [Preprint]. 2024 Nov 22:2024.11.21.24317744. doi: 10.1101/2024.11.21.24317744. medRxiv. 2024. PMID: 39606390 Free PMC article. Preprint.
PAM-free diagnostics with diverse type V CRISPR-Cas systems.
Rananaware SR, Meister KS, Shoemaker GM, Vesco EK, Sandoval LSW, Lewis JG, Bodin AP, Karalkar VN, Lange IH, Pizzano BLM, Chang M, Ahmadimashhadi MR, Flannery SJ, Nguyen LT, Wang GP, Jain PK. Rananaware SR, et al. medRxiv [Preprint]. 2024 May 3:2024.05.02.24306194. doi: 10.1101/2024.05.02.24306194. medRxiv. 2024. PMID: 38746294 Free PMC article. Preprint.
Engineering highly thermostable Cas12b via de novo structural analyses for one-pot detection of nucleic acids.
Nguyen LT, Rananaware SR, Yang LG, Macaluso NC, Ocana-Ortiz JE, Meister KS, Pizzano BLM, Sandoval LSW, Hautamaki RC, Fang ZR, Joseph SM, Shoemaker GM, Carman DR, Chang L, Rakestraw NR, Zachary JF, Guerra S, Perez A, Jain PK. Nguyen LT, et al. Cell Rep Med. 2023 May 16;4(5):101037. doi: 10.1016/j.xcrm.2023.101037. Epub 2023 May 8. Cell Rep Med. 2023. PMID: 37160120 Free PMC article.