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Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases.
Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS. Yarnall MTN, et al. Nat Biotechnol. 2023 Apr;41(4):500-512. doi: 10.1038/s41587-022-01527-4. Epub 2022 Nov 24. Nat Biotechnol. 2023. PMID: 36424489 Free PMC article.
Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing.
Joung J, Ladha A, Saito M, Kim NG, Woolley AE, Segel M, Barretto RPJ, Ranu A, Macrae RK, Faure G, Ioannidi EI, Krajeski RN, Bruneau R, Huang MW, Yu XG, Li JZ, Walker BD, Hung DT, Greninger AL, Jerome KR, Gootenberg JS, Abudayyeh OO, Zhang F. Joung J, et al. N Engl J Med. 2020 Oct 8;383(15):1492-1494. doi: 10.1056/NEJMc2026172. Epub 2020 Sep 16. N Engl J Med. 2020. PMID: 32937062 Free PMC article. No abstract available.
Rapid protein evolution by few-shot learning with a protein language model.
Jiang K, Yan Z, Di Bernardo M, Sgrizzi SR, Villiger L, Kayabolen A, Kim B, Carscadden JK, Hiraizumi M, Nishimasu H, Gootenberg JS, Abudayyeh OO. Jiang K, et al. bioRxiv [Preprint]. 2024 Jul 18:2024.07.17.604015. doi: 10.1101/2024.07.17.604015. bioRxiv. 2024. PMID: 39071429 Free PMC article. Preprint.