Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation

Search Page

Filters

My Custom Filters

Results by year

Table representation of search results timeline featuring number of search results per year.

Year Number of Results
2020 3
2021 4
2023 4
2024 1
2025 0

Publication date

Text availability

Article attribute

Article type

Additional filters

Article Language

Species

Sex

Age

Other

Search Results

11 results

Results by year

Filters applied: . Clear all
Your search was processed without automatic term mapping because it retrieved zero results.
Page 1
2,3-Dihydroxyisovalerate production by Klebsiella pneumoniae.
Wang Y, Gu J, Lu X, Zhang Z, Yang Y, Sun S, Kostas ET, Shi J, Gao M, Baganz F, Lye GJ, Hao J. Wang Y, et al. Appl Microbiol Biotechnol. 2020 Aug;104(15):6601-6613. doi: 10.1007/s00253-020-10711-y. Epub 2020 Jun 9. Appl Microbiol Biotechnol. 2020. PMID: 32519119
Thus, a biological route of 2,3-dihydroxyisovalerate production with high conversion ratio and final titer was developed, providing a basis for an industrial process. Key Points A biological route of 2,3-dihydroxyisovalerate production was setup. Disruption of budA causes …
Thus, a biological route of 2,3-dihydroxyisovalerate production with high conversion ratio and final titer was developed, providing a basis …
Production of 21-hydroxy-20-methyl-pregna-1,4-dien-3-one by modifying multiple genes in Mycolicibacterium.
Yuan C, Ma Z, Li Y, Zhang J, Liu X, Han S, Du G, Shi J, Sun J, Zhang B. Yuan C, et al. Appl Microbiol Biotechnol. 2023 Mar;107(5-6):1563-1574. doi: 10.1007/s00253-023-12399-2. Epub 2023 Feb 2. Appl Microbiol Biotechnol. 2023. PMID: 36729227
The molar yield and the purity of 1,4-HP were optimal so far, and the production of 1,4-HP provides a new intermediate for the pharmaceutical steroid industry. KEY POINTS: A third 3-ketosteroid-9-hydroxylase was identified in Mycolicibacterium neoaurum. ...
The molar yield and the purity of 1,4-HP were optimal so far, and the production of 1,4-HP provides a new intermediate for the pharmaceutica …
Functional analysis of acyl-CoA dehydrogenases and their application to C22 steroid production.
Yuan C, Li Y, Han S, He B, Zhai X, Lin W, Shi J, Sun J, Zhang B. Yuan C, et al. Appl Microbiol Biotechnol. 2023 Jun;107(11):3419-3428. doi: 10.1007/s00253-023-12541-0. Epub 2023 Apr 24. Appl Microbiol Biotechnol. 2023. PMID: 37093308
This study improved the understanding of the steroid bioconversion pathway and proposed a method of the production of a new C22 steroid. KEY POINTS: Three ChsE paralogs from M. neoaurum are identified and studied. The function of ChsE is overlapped in vivo. ...
This study improved the understanding of the steroid bioconversion pathway and proposed a method of the production of a new C22 steroid. …
Whole-Genome Analysis of Mycobacterium neoaurum DSM 1381 and the Validation of Two Key Enzymes Affecting C22 Steroid Intermediates in Sterol Metabolism.
Zhang J, Zhang R, Song S, Su Z, Shi J, Cao H, Zhang B. Zhang J, et al. Int J Mol Sci. 2023 Mar 24;24(7):6148. doi: 10.3390/ijms24076148. Int J Mol Sci. 2023. PMID: 37047121 Free PMC article.
The 3D models of these two enzymes were predicted by SWISS-MODEL and AlphaFold2 to understand the inactivation of the two key enzymes. These results indicate that K171E in Hsd4A may destroy the inaction between the NAD+ with the NH3+ and N173D in KshA1 and may disrupt the …
The 3D models of these two enzymes were predicted by SWISS-MODEL and AlphaFold2 to understand the inactivation of the two key enzymes …
1,2-Propanediol production from glycerol via an endogenous pathway of Klebsiella pneumoniae.
Sun S, Shu L, Lu X, Wang Q, Tišma M, Zhu C, Shi J, Baganz F, Lye GJ, Hao J. Sun S, et al. Appl Microbiol Biotechnol. 2021 Dec;105(23):9003-9016. doi: 10.1007/s00253-021-11652-w. Epub 2021 Nov 8. Appl Microbiol Biotechnol. 2021. PMID: 34748036
But this strain still utilized glycerol, and 1,2-propanediol became the main catabolite. Key enzymes of 1,2-propanediol synthesis from glycerol were investigated in detail. dhaD and gldA encoded glycerol dehydrogenases were both responsible for the conversion of glycerol t …
But this strain still utilized glycerol, and 1,2-propanediol became the main catabolite. Key enzymes of 1,2-propanediol synthesis fro …
Improving the production of 9α-hydroxy-4-androstene-3,17-dione from phytosterols by 3-ketosteroid-Δ1-dehydrogenase deletions and multiple genetic modifications in Mycobacterium fortuitum.
Liu X, Zhang J, Yuan C, Du G, Han S, Shi J, Sun J, Zhang B. Liu X, et al. Microb Cell Fact. 2023 Mar 16;22(1):53. doi: 10.1186/s12934-023-02052-y. Microb Cell Fact. 2023. PMID: 36922830 Free PMC article.
CONCLUSIONS: KstD2 and KstD3 are the main dehydrogenases that lead to 9-OHAD degradation. Hsd4A and Opccr are key enzymes regulating the metabolic flux of the C19- and C22-pathways. Overexpression of fadE28-29 can reduce the accumulation of incomplete degradation products …
CONCLUSIONS: KstD2 and KstD3 are the main dehydrogenases that lead to 9-OHAD degradation. Hsd4A and Opccr are key enzymes regulating …
Efficient Production of 9,22-Dihydroxy-23,24-bisnorchol-4-ene-3-one from Phytosterols by Modifying Multiple Genes in Mycobacterium fortuitum.
Han S, Liu X, He B, Zhai X, Yuan C, Li Y, Lin W, Wang H, Zhang B. Han S, et al. Int J Mol Sci. 2024 Mar 22;25(7):3579. doi: 10.3390/ijms25073579. Int J Mol Sci. 2024. PMID: 38612391 Free PMC article.
We used a mutant strain, MFdeltakstD, that knocked out kstds from Mycobacterium fortuitum ATCC 35855 named MFKD in this study as the original strain. Hsd4A and FadA5 are key enzymes in controlling the C19 metabolic pathway of steroids in Mycobacterium fortuitum ATCC 35855. …
We used a mutant strain, MFdeltakstD, that knocked out kstds from Mycobacterium fortuitum ATCC 35855 named MFKD in this study as the origina …
11 results