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An efficient method to express GPI-anchor proteins in insect cells.
Shams-Eldin H, Azzouz N, Niehus S, Smith TK, Schwarz RT. Shams-Eldin H, et al. Biochem Biophys Res Commun. 2008 Jan 25;365(4):657-63. doi: 10.1016/j.bbrc.2007.11.026. Epub 2007 Nov 20. Biochem Biophys Res Commun. 2008. PMID: 18029261
Toxoplasma gondii grown in human cells uses GalNAc-containing glycosylphosphatidylinositol precursors to anchor surface antigens while the immunogenic Glc-GalNAc-containing precursors remain free at the parasite cell surface.
Azzouz N, Shams-Eldin H, Niehus S, Debierre-Grockiego F, Bieker U, Schmidt J, Mercier C, Delauw MF, Dubremetz JF, Smith TK, Schwarz RT. Azzouz N, et al. Int J Biochem Cell Biol. 2006;38(11):1914-25. doi: 10.1016/j.biocel.2006.05.006. Epub 2006 May 27. Int J Biochem Cell Biol. 2006. PMID: 16822699