Two-step freezing procedure for cryopreservation of rumen ciliates, an effective tool for creation of a frozen rumen protozoa bank.
Nsabimana E, Kisidayová S, Macheboeuf D, Newbold CJ, Jouany JP.
Nsabimana E, et al. Among authors: kisidayova s.
Appl Environ Microbiol. 2003 Jul;69(7):3826-32. doi: 10.1128/AEM.69.7.3826-3832.2003.
Appl Environ Microbiol. 2003.
PMID: 12839750
Free PMC article.
In addition to the nature of the cryoprotectant (dimethyl sulfoxide), the equilibration temperature and equilibration time (25 degrees C and 5 min, respectively), and the holding time at subzero temperature (45 min) recommended previously (S. Kisidayova, J. Microbio …
In addition to the nature of the cryoprotectant (dimethyl sulfoxide), the equilibration temperature and equilibration time (25 degrees C and …