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A polyclonal antibody preparation with Michaelian catalytic properties.
Gallacher G, Jackson CS, Searcey M, Badman GT, Goel R, Topham CM, Mellor GW, Brocklehurst K. Gallacher G, et al. Among authors: mellor gw. Biochem J. 1991 Nov 1;279 ( Pt 3)(Pt 3):871-81. doi: 10.1042/bj2790871. Biochem J. 1991. PMID: 1953683 Free PMC article.
Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.
Thomas MP, Topham CM, Kowlessur D, Mellor GW, Thomas EW, Whitford D, Brocklehurst K. Thomas MP, et al. Among authors: mellor gw. Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805. Biochem J. 1994. PMID: 8010964 Free PMC article.
Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu.
Mellor GW, Patel M, Thomas EW, Brocklehurst K. Mellor GW, et al. Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):201-10. doi: 10.1042/bj2940201. Biochem J. 1993. PMID: 8103322 Free PMC article.
28 results