DNA-DNA subtractive cDNA cloning using oligo(dT)30-Latex and PCR: identification of cellular genes which are overexpressed in senescent human diploid fibroblasts.
Hara E, Yamaguchi T, Tahara H, Tsuyama N, Tsurui H, Ide T, Oda K.
Hara E, et al.
Anal Biochem. 1993 Oct;214(1):58-64. doi: 10.1006/abio.1993.1456.
Anal Biochem. 1993.
PMID: 8250255
We previously reported an efficient method for subtractive cDNA cloning using oligo(dT)30-Latex and polymerase chain reaction (PCR) (E. Hara et al., Nucleic Acids Res. 19, 7097-7104, 1991). The subtraction was performed by hybridization between mRNA of cell type B a …
We previously reported an efficient method for subtractive cDNA cloning using oligo(dT)30-Latex and polymerase chain reaction (PCR) (E …