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Solution structure and function in trifluoroethanol of PP-50, an ATP-binding peptide from F1ATPase.
Chuang WJ, Abeygunawardana C, Gittis AG, Pedersen PL, Mildvan AS. Chuang WJ, et al. Among authors: mildvan as. Arch Biochem Biophys. 1995 May 10;319(1):110-22. doi: 10.1006/abbi.1995.1272. Arch Biochem Biophys. 1995. PMID: 7771774 Free article.
Abeygunawardana, P. L. Pedersen, and A. S. Mildvan, 1992, Biochemistry 31, 7915-7921). CD titration of PP-50 with trifluoroethanol (TFE) reveals a decrease in ellipticity at 208 and 222 nm, saturating at 25% TFE. ...In H2O the binding of H2ATP2- increases the structure of …
Abeygunawardana, P. L. Pedersen, and A. S. Mildvan, 1992, Biochemistry 31, 7915-7921). CD titration of PP-50 with trifluoroethanol (T …
NMR docking of a substrate into the X-ray structure of the Asp-21-->Glu mutant of staphylococcal nuclease.
Weber DJ, Libson AM, Gittis AG, Lebowitz MS, Mildvan AS. Weber DJ, et al. Among authors: mildvan as. Biochemistry. 1994 Jul 5;33(26):8017-28. doi: 10.1021/bi00192a005. Biochemistry. 1994. PMID: 8025106
The Co2+ to 31P distance of 4.1 +/- 0.9 A agrees with that found on the wild-type enzyme (Weber, D. J., Mullen, G. P., & Mildvan, A. S. (1991) Biochemistry 30, 7425-7437) and indicates at least 18% inner sphere phosphate coordination. ...Both the metal-nucleus and inte …
The Co2+ to 31P distance of 4.1 +/- 0.9 A agrees with that found on the wild-type enzyme (Weber, D. J., Mullen, G. P., & Mildvan, …
Catalytic role of the amino-terminal proline in 4-oxalocrotonate tautomerase: affinity labeling and heteronuclear NMR studies.
Stivers JT, Abeygunawardana C, Mildvan AS, Hajipour G, Whitman CP, Chen LH. Stivers JT, et al. Among authors: mildvan as. Biochemistry. 1996 Jan 23;35(3):803-13. doi: 10.1021/bi951077g. Biochemistry. 1996. PMID: 8547260
Finally, the pH dependence of kinact/KI for affinity labeling by 3-BP yields a pKa value of 6.7 +/- 0.3, in reasonable agreement with the pKa values found for kcat/KM for the non-sticky substrate 2-hydroxy-2,4-pentadienoate and by direct NMR titration of Pro-1 [Stivers, J. T., Ab …
Finally, the pH dependence of kinact/KI for affinity labeling by 3-BP yields a pKa value of 6.7 +/- 0.3, in reasonable agreement with the pK …
222 results