Protocol to measure human IL-6 secretion from CAR T cell-primed macrophage and monocyte lineage cells in vitro and in vivo using humanized mice.
Nguyen T, Yoshikawa T, Ito Y, Kasuya H, Nakashima T, Okamoto S, Amaishi Y, Zhang H, Li Y, Matsukawa T, Inoue S, Kagoya Y.
Nguyen T, et al. Among authors: yoshikawa t.
STAR Protoc. 2024 Dec 20;5(4):103423. doi: 10.1016/j.xpro.2024.103423. Epub 2024 Nov 1.
STAR Protoc. 2024.
PMID: 39488831
Free PMC article.
Here, we describe a protocol to generate anti-CD19 CAR T cells and quantify human monocyte-derived IL-6 cocultured with CAR T cells and target tumor cells in vitro. We further describe a protocol to generate a humanized mouse model and evaluate CAR T cell-ass …
Here, we describe a protocol to generate anti-CD19 CAR T cells and quantify human monocyte-derived IL-6 cocultured with CAR T …