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Time-resolved serial crystallography to track the dynamics of carbon monoxide in the active site of cytochrome c oxidase.
Safari C, Ghosh S, Andersson R, Johannesson J, Båth P, Uwangue O, Dahl P, Zoric D, Sandelin E, Vallejos A, Nango E, Tanaka R, Bosman R, Börjesson P, Dunevall E, Hammarin G, Ortolani G, Panman M, Tanaka T, Yamashita A, Arima T, Sugahara M, Suzuki M, Masuda T, Takeda H, Yamagiwa R, Oda K, Fukuda M, Tosha T, Naitow H, Owada S, Tono K, Nureki O, Iwata S, Neutze R, Brändén G. Safari C, et al. Among authors: tosha t. Sci Adv. 2023 Dec 8;9(49):eadh4179. doi: 10.1126/sciadv.adh4179. Epub 2023 Dec 8. Sci Adv. 2023. PMID: 38064560 Free PMC article.
Dynamics of nitric oxide controlled by protein complex in bacterial system.
Terasaka E, Yamada K, Wang PH, Hosokawa K, Yamagiwa R, Matsumoto K, Ishii S, Mori T, Yagi K, Sawai H, Arai H, Sugimoto H, Sugita Y, Shiro Y, Tosha T. Terasaka E, et al. Among authors: tosha t. Proc Natl Acad Sci U S A. 2017 Sep 12;114(37):9888-9893. doi: 10.1073/pnas.1621301114. Epub 2017 Aug 28. Proc Natl Acad Sci U S A. 2017. PMID: 28847930 Free PMC article.
Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate.
Tosha T, Nomura T, Nishida T, Saeki N, Okubayashi K, Yamagiwa R, Sugahara M, Nakane T, Yamashita K, Hirata K, Ueno G, Kimura T, Hisano T, Muramoto K, Sawai H, Takeda H, Mizohata E, Yamashita A, Kanematsu Y, Takano Y, Nango E, Tanaka R, Nureki O, Shoji O, Ikemoto Y, Murakami H, Owada S, Tono K, Yabashi M, Yamamoto M, Ago H, Iwata S, Sugimoto H, Shiro Y, Kubo M. Tosha T, et al. Nat Commun. 2017 Nov 17;8(1):1585. doi: 10.1038/s41467-017-01702-1. Nat Commun. 2017. PMID: 29147002 Free PMC article.
Serial Femtosecond Zero Dose Crystallography Captures a Water-Free Distal Heme Site in a Dye-Decolorising Peroxidase to Reveal a Catalytic Role for an Arginine in FeIV =O Formation.
Lučić M, Svistunenko DA, Wilson MT, Chaplin AK, Davy B, Ebrahim A, Axford D, Tosha T, Sugimoto H, Owada S, Dworkowski FSN, Tews I, Owen RL, Hough MA, Worrall JAR. Lučić M, et al. Among authors: tosha t. Angew Chem Int Ed Engl. 2020 Nov 23;59(48):21656-21662. doi: 10.1002/anie.202008622. Epub 2020 Sep 23. Angew Chem Int Ed Engl. 2020. PMID: 32780931 Free PMC article.
Short-lived intermediate in N2O generation by P450 NO reductase captured by time-resolved IR spectroscopy and XFEL crystallography.
Nomura T, Kimura T, Kanematsu Y, Yamada D, Yamashita K, Hirata K, Ueno G, Murakami H, Hisano T, Yamagiwa R, Takeda H, Gopalasingam C, Kousaka R, Yanagisawa S, Shoji O, Kumasaka T, Yamamoto M, Takano Y, Sugimoto H, Tosha T, Kubo M, Shiro Y. Nomura T, et al. Among authors: tosha t. Proc Natl Acad Sci U S A. 2021 May 25;118(21):e2101481118. doi: 10.1073/pnas.2101481118. Proc Natl Acad Sci U S A. 2021. PMID: 34001620 Free PMC article.
Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature.
Moreno-Chicano T, Carey LM, Axford D, Beale JH, Doak RB, Duyvesteyn HME, Ebrahim A, Henning RW, Monteiro DCF, Myles DA, Owada S, Sherrell DA, Straw ML, Šrajer V, Sugimoto H, Tono K, Tosha T, Tews I, Trebbin M, Strange RW, Weiss KL, Worrall JAR, Meilleur F, Owen RL, Ghiladi RA, Hough MA. Moreno-Chicano T, et al. Among authors: tosha t. IUCrJ. 2022 Jul 25;9(Pt 5):610-624. doi: 10.1107/S2052252522006418. eCollection 2022 Sep 1. IUCrJ. 2022. PMID: 36071813 Free PMC article.
Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism.
Fadini A, Hutchison CDM, Morozov D, Chang J, Maghlaoui K, Perrett S, Luo F, Kho JCX, Romei MG, Morgan RML, Orr CM, Cordon-Preciado V, Fujiwara T, Nuemket N, Tosha T, Tanaka R, Owada S, Tono K, Iwata S, Boxer SG, Groenhof G, Nango E, van Thor JJ. Fadini A, et al. Among authors: tosha t. J Am Chem Soc. 2023 Jul 26;145(29):15796-15808. doi: 10.1021/jacs.3c02313. Epub 2023 Jul 7. J Am Chem Soc. 2023. PMID: 37418747 Free PMC article.
84 results