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Addressing the dNTP bottleneck restricting prime editing activity.
Ponnienselvan K, Liu P, Nyalile T, Oikemus S, Joynt AT, Kelly K, Guo D, Chen Z, Lee JM, Schiffer CA, Emerson CP, Lawson ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA. Ponnienselvan K, et al. Among authors: chen z. bioRxiv [Preprint]. 2023 Oct 29:2023.10.21.563443. doi: 10.1101/2023.10.21.563443. bioRxiv. 2023. PMID: 37904991 Free PMC article. Preprint.
5'-Modifications improve potency and efficacy of DNA donors for precision genome editing.
Ghanta KS, Chen Z, Mir A, Dokshin GA, Krishnamurthy PM, Yoon Y, Gallant J, Xu P, Zhang XO, Ozturk AR, Shin M, Idrizi F, Liu P, Gneid H, Edraki A, Lawson ND, Rivera-Pérez JA, Sontheimer EJ, Watts JK, Mello CC. Ghanta KS, et al. Among authors: chen z. Elife. 2021 Oct 19;10:e72216. doi: 10.7554/eLife.72216. Elife. 2021. PMID: 34665130 Free PMC article.
Tetrazine-Ligated CRISPR sgRNAs for Efficient Genome Editing.
Chen Z, Devi G, Arif A, Zamore PD, Sontheimer EJ, Watts JK. Chen Z, et al. ACS Chem Biol. 2022 May 20;17(5):1045-1050. doi: 10.1021/acschembio.2c00116. Epub 2022 Apr 21. ACS Chem Biol. 2022. PMID: 35446558 Free PMC article.
Self-delivering CRISPR RNAs for AAV Co-delivery and Genome Editing in vivo.
Zhang H, Kelly K, Lee J, Echeverria D, Cooper D, Panwala R, Chen Z, Gaston N, Newby GA, Xie J, Liu DR, Gao G, Wolfe SA, Khvorova A, Watts JK, Sontheimer EJ. Zhang H, et al. Among authors: chen z. bioRxiv [Preprint]. 2023 Mar 23:2023.03.20.533459. doi: 10.1101/2023.03.20.533459. bioRxiv. 2023. Update in: Nucleic Acids Res. 2024 Jan 25;52(2):977-997. doi: 10.1093/nar/gkad1125 PMID: 36993169 Free PMC article. Updated. Preprint.
Increasing intracellular dNTP levels improves prime editing efficiency.
Liu P, Ponnienselvan K, Nyalile T, Oikemus S, Joynt AT, Iyer S, Kelly K, Guo D, Kyawe PP, Vanderleeden E, Redick SD, Huang L, Chen Z, Lee JM, Schiffer CA, Harlan DM, Wang JP, Emerson CP Jr, Lawson ND, Watts JK, Sontheimer EJ, Luban J, Wolfe SA. Liu P, et al. Among authors: chen z. Nat Biotechnol. 2024 Sep 25. doi: 10.1038/s41587-024-02405-x. Online ahead of print. Nat Biotechnol. 2024. PMID: 39322763
76,008 results
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