Modular pooled discovery of synthetic knockin sequences to program durable cell therapies.
Blaeschke F, Chen YY, Apathy R, Daniel B, Chen AY, Chen PA, Sandor K, Zhang W, Li Z, Mowery CT, Yamamoto TN, Nyberg WA, To A, Yu R, Bueno R, Kim MC, Schmidt R, Goodman DB, Feuchtinger T, Eyquem J, Jimmie Ye C, Carnevale J, Satpathy AT, Shifrut E, Roth TL, Marson A.
Blaeschke F, et al. Among authors: to a.
Cell. 2023 Sep 14;186(19):4216-4234.e33. doi: 10.1016/j.cell.2023.08.013.
Cell. 2023.
PMID: 37714135
Free PMC article.
ModPoKI's modularity allowed us to generate an 10,000-member library of TF combinations. Non-viral KI of a combined BATF-TFAP4 polycistronic construct enhanced fitness. Overexpressed BATF and TFAP4 co-occupy and regulate key gene targets to reprogram T cell f …
ModPoKI's modularity allowed us to generate an 10,000-member library of TF combinations. Non-viral KI of a combined BATF-TFAP4 …