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CASPON platform technology: Ultrafast circularly permuted caspase-2 cleaves tagged fusion proteins before all 20 natural amino acids at the N-terminus.
Lingg N, Kröß C, Engele P, Öhlknecht C, Köppl C, Fischer A, Lier B, Loibl J, Sprenger B, Liu J, Scheidl P, Berkemeyer M, Buchinger W, Brocard C, Striedner G, Oostenbrink C, Schneider R, Jungbauer A, Cserjan-Puschmann M. Lingg N, et al. Among authors: engele p. N Biotechnol. 2022 Nov 25;71:37-46. doi: 10.1016/j.nbt.2022.07.002. Epub 2022 Aug 1. N Biotechnol. 2022. PMID: 35926774 Free article.
Production of Circularly Permuted Caspase-2 for Affinity Fusion-Tag Removal: Cloning, Expression in Escherichia coli, Purification, and Characterization.
Cserjan-Puschmann M, Lingg N, Engele P, Kröß C, Loibl J, Fischer A, Bacher F, Frank AC, Öhlknecht C, Brocard C, Oostenbrink C, Berkemeyer M, Schneider R, Striedner G, Jungbauer A. Cserjan-Puschmann M, et al. Among authors: engele p. Biomolecules. 2020 Nov 24;10(12):1592. doi: 10.3390/biom10121592. Biomolecules. 2020. PMID: 33255244 Free PMC article.
PROFICS: A bacterial selection system for directed evolution of proteases.
Kröß C, Engele P, Sprenger B, Fischer A, Lingg N, Baier M, Öhlknecht C, Lier B, Oostenbrink C, Cserjan-Puschmann M, Striedner G, Jungbauer A, Schneider R. Kröß C, et al. Among authors: engele p. J Biol Chem. 2021 Oct;297(4):101095. doi: 10.1016/j.jbc.2021.101095. Epub 2021 Aug 19. J Biol Chem. 2021. PMID: 34418435 Free PMC article.