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Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment.
Salih E, Malthouse JP, Kowlessur D, Jarvis M, O'Driscoll M, Brocklehurst K. Salih E, et al. Among authors: brocklehurst k. Biochem J. 1987 Oct 1;247(1):181-93. doi: 10.1042/bj2470181. Biochem J. 1987. PMID: 2825655 Free PMC article.
Investigation of the catalytic site of actinidin by using benzofuroxan as a reactivity probe with selectivity for the thiolate-imidazolium ion-pair systems of cysteine proteinases. Evidence that the reaction of the ion-pair of actinidin (pKI 3.0, pKII 9.6) is modulated by the state of ionization of a group associated with a molecular pKa of 5.5.
Salih E, Brocklehurst K. Salih E, et al. Among authors: brocklehurst k. Biochem J. 1983 Sep 1;213(3):713-8. doi: 10.1042/bj2130713. Biochem J. 1983. PMID: 6311173 Free PMC article.
In marked contrast with the analogous reaction of papain (reported by Shipton & Brocklehurst [(1977) Biochem. J. 167, 799-810] ) the pH-k profile for the actinidin reaction clearly contains a sigmoidal component with pKa 5.5, in which k increases with dec …
In marked contrast with the analogous reaction of papain (reported by Shipton & Brocklehurst [(1977) Biochem. J. 167, 799-810] ) …
'Chymopapain S' is chymopapain A.
Brocklehurst K, Baines BS, Salih E, Hatzoulis C. Brocklehurst K, et al. Biochem J. 1984 Jul 15;221(2):553-4. doi: 10.1042/bj2210553. Biochem J. 1984. PMID: 6383349 Free PMC article. No abstract available.
Characterization of papaya peptidase A as a cysteine proteinase of Carica papaya L. with active-centre properties that differ from those of papain by using 2,2'-dipyridyl disulphide and 4-chloro-7-nitrobenzofurazan as reactivity probes. Use of two-protonic-state electrophiles in the identification of catalytic-site thiol groups.
Baines BS, Brocklehurst K. Baines BS, et al. Among authors: brocklehurst k. Biochem J. 1982 Jul 1;205(1):205-11. doi: 10.1042/bj2050205. Biochem J. 1982. PMID: 6751321 Free PMC article.
203 results