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A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations.
Bertoglio F, Fühner V, Ruschig M, Heine PA, Abassi L, Klünemann T, Rand U, Meier D, Langreder N, Steinke S, Ballmann R, Schneider KT, Roth KDR, Kuhn P, Riese P, Schäckermann D, Korn J, Koch A, Chaudhry MZ, Eschke K, Kim Y, Zock-Emmenthal S, Becker M, Scholz M, Moreira GMSG, Wenzel EV, Russo G, Garritsen HSP, Casu S, Gerstner A, Roth G, Adler J, Trimpert J, Hermann A, Schirrmann T, Dübel S, Frenzel A, Van den Heuvel J, Čičin-Šain L, Schubert M, Hust M. Bertoglio F, et al. Among authors: schackermann d. Cell Rep. 2021 Jul 27;36(4):109433. doi: 10.1016/j.celrep.2021.109433. Epub 2021 Jul 7. Cell Rep. 2021. PMID: 34273271 Free PMC article.
Baculovirus-free insect cell expression system for high yield antibody and antigen production.
Korn J, Schäckermann D, Kirmann T, Bertoglio F, Steinke S, Heisig J, Ruschig M, Rojas G, Langreder N, Wenzel EV, Roth KDR, Becker M, Meier D, van den Heuvel J, Hust M, Dübel S, Schubert M. Korn J, et al. Among authors: schackermann d. Sci Rep. 2020 Dec 7;10(1):21393. doi: 10.1038/s41598-020-78425-9. Sci Rep. 2020. PMID: 33288836 Free PMC article.
SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface.
Bertoglio F, Meier D, Langreder N, Steinke S, Rand U, Simonelli L, Heine PA, Ballmann R, Schneider KT, Roth KDR, Ruschig M, Riese P, Eschke K, Kim Y, Schäckermann D, Pedotti M, Kuhn P, Zock-Emmenthal S, Wöhrle J, Kilb N, Herz T, Becker M, Grasshoff M, Wenzel EV, Russo G, Kröger A, Brunotte L, Ludwig S, Fühner V, Krämer SD, Dübel S, Varani L, Roth G, Čičin-Šain L, Schubert M, Hust M. Bertoglio F, et al. Among authors: schackermann d. Nat Commun. 2021 Mar 11;12(1):1577. doi: 10.1038/s41467-021-21609-2. Nat Commun. 2021. PMID: 33707427 Free PMC article.
Antibody Affinity and Stability Maturation by Error-Prone PCR.
Langreder N, Schäckermann D, Unkauf T, Schubert M, Frenzel A, Bertoglio F, Hust M. Langreder N, et al. Among authors: schackermann d. Methods Mol Biol. 2023;2702:395-410. doi: 10.1007/978-1-0716-3381-6_20. Methods Mol Biol. 2023. PMID: 37679631