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Enzymatic methyl sequencing detects DNA methylation at single-base resolution from picograms of DNA.
Vaisvila R, Ponnaluri VKC, Sun Z, Langhorst BW, Saleh L, Guan S, Dai N, Campbell MA, Sexton BS, Marks K, Samaranayake M, Samuelson JC, Church HE, Tamanaha E, Corrêa IR Jr, Pradhan S, Dimalanta ET, Evans TC Jr, Williams L, Davis TB. Vaisvila R, et al. Among authors: saleh l. Genome Res. 2021 Jul;31(7):1280-1289. doi: 10.1101/gr.266551.120. Epub 2021 Jun 17. Genome Res. 2021. PMID: 34140313 Free PMC article.
Nondestructive enzymatic deamination enables single-molecule long-read amplicon sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution.
Sun Z, Vaisvila R, Hussong LM, Yan B, Baum C, Saleh L, Samaranayake M, Guan S, Dai N, Corrêa IR Jr, Pradhan S, Davis TB, Evans TC Jr, Ettwiller LM. Sun Z, et al. Among authors: saleh l. Genome Res. 2021 Feb;31(2):291-300. doi: 10.1101/gr.265306.120. Epub 2021 Jan 19. Genome Res. 2021. PMID: 33468551 Free PMC article.
Biochemical characterization of a Naegleria TET-like oxygenase and its application in single molecule sequencing of 5-methylcytosine.
Pais JE, Dai N, Tamanaha E, Vaisvila R, Fomenkov AI, Bitinaite J, Sun Z, Guan S, Corrêa IR Jr, Noren CJ, Cheng X, Roberts RJ, Zheng Y, Saleh L. Pais JE, et al. Among authors: saleh l. Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4316-21. doi: 10.1073/pnas.1417939112. Epub 2015 Mar 23. Proc Natl Acad Sci U S A. 2015. PMID: 25831492 Free PMC article.
Phage-encoded ten-eleven translocation dioxygenase (TET) is active in C5-cytosine hypermodification in DNA.
Burke EJ, Rodda SS, Lund SR, Sun Z, Zeroka MR, O'Toole KH, Parker MJ, Doshi DS, Guan C, Lee YJ, Dai N, Hough DM, Shnider DA, Corrêa IR Jr, Weigele PR, Saleh L. Burke EJ, et al. Among authors: saleh l. Proc Natl Acad Sci U S A. 2021 Jun 29;118(26):e2026742118. doi: 10.1073/pnas.2026742118. Proc Natl Acad Sci U S A. 2021. PMID: 34155108 Free PMC article.
Pathways of thymidine hypermodification.
Lee YJ, Dai N, Müller SI, Guan C, Parker MJ, Fraser ME, Walsh SE, Sridar J, Mulholland A, Nayak K, Sun Z, Lin YC, Comb DG, Marks K, Gonzalez R, Dowling DP, Bandarian V, Saleh L, Corrêa IR, Weigele PR. Lee YJ, et al. Among authors: saleh l. Nucleic Acids Res. 2022 Apr 8;50(6):3001-3017. doi: 10.1093/nar/gkab781. Nucleic Acids Res. 2022. PMID: 34522950 Free PMC article.
316 results