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Refolding of [6-19F]tryptophan-labeled Escherichia coli dihydrofolate reductase in the presence of ligand: a stopped-flow NMR spectroscopy study.
Hoeltzli SD, Frieden C. Hoeltzli SD, et al. Among authors: frieden c. Biochemistry. 1998 Jan 6;37(1):387-98. doi: 10.1021/bi971962u. Biochemistry. 1998. PMID: 9425060
Using site-directed mutagenesis, these resonances have been assigned to individual tryptophans [Hoeltzli, S. D., and Frieden, C. (1994) Biochemistry 33, 5502-5509], allowing both the native and unfolded environments of each tryptophan to be monitored during the refo …
Using site-directed mutagenesis, these resonances have been assigned to individual tryptophans [Hoeltzli, S. D., and Frieden, C …
NMR studies of 4-19F-phenylalanine-labeled intestinal fatty acid binding protein: evidence for conformational heterogeneity in the native state.
Li H, Frieden C. Li H, et al. Among authors: frieden c. Biochemistry. 2005 Feb 22;44(7):2369-77. doi: 10.1021/bi047600l. Biochemistry. 2005. PMID: 15709749
There are 29 residues involved in van der Waals or hydrophobic interactions or both to form a U-shaped ligand binding pocket (Sacchettni J. C., Scapin G., Gopaul D., and Gordon J. I. (1992) J. Biol. Chem. 267, 23534-23545). ...Phe47, Phe62, Phe68, Phe93, and Trp82 have bee …
There are 29 residues involved in van der Waals or hydrophobic interactions or both to form a U-shaped ligand binding pocket (Sacchettni J. …
Phenylalanine side chain behavior of the intestinal fatty acid-binding protein: the effect of urea on backbone and side chain stability.
Li H, Frieden C. Li H, et al. Among authors: frieden c. J Biol Chem. 2005 Nov 18;280(46):38556-61. doi: 10.1074/jbc.M505435200. Epub 2005 Sep 14. J Biol Chem. 2005. PMID: 16162507 Free article.
The results were compared with previous studies examining the backbone behavior (Hodsdon, M. E., and Frieden, C. (2001) Biochemistry 40, 732-742) and suggest that the side chains show more stability than the backbone prior to global unfolding of the protein....
The results were compared with previous studies examining the backbone behavior (Hodsdon, M. E., and Frieden, C. (2001) Bioche …
211 results