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Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.
Brocklehurst K, Brocklehurst SM, Kowlessur D, O'Driscoll M, Patel G, Salih E, Templeton W, Thomas E, Topham CM, Willenbrock F. Brocklehurst K, et al. Among authors: brocklehurst sm. Biochem J. 1988 Dec 1;256(2):543-58. doi: 10.1042/bj2560543. Biochem J. 1988. PMID: 3223929 Free PMC article.
The reactivities of the two enzymes towards the four reactivity probes (I)-(IV) and also that of papain towards 2-(N'-acetyl-L-phenylalanylamino)ethyl 2'-pyridyl disulphide (VII) (containing both a P1-P2 amide bond and an L-phenylalanyl side chain as an occupant for the S2 subsit …
The reactivities of the two enzymes towards the four reactivity probes (I)-(IV) and also that of papain towards 2-(N'-acetyl-L-phenylalanyla …
Evaluation of hydrogen-bonding and enantiomeric P2-S2 hydrophobic contacts in dynamic aspects of molecular recognition by papain.
Patel M, Kayani IS, Templeton W, Mellor GW, Thomas EW, Brocklehurst K. Patel M, et al. Among authors: brocklehurst k. Biochem J. 1992 Nov 1;287 ( Pt 3)(Pt 3):881-9. doi: 10.1042/bj2870881. Biochem J. 1992. PMID: 1445247 Free PMC article.
The results of a study of the pH-dependence of the second-order rate constant (k) for its reaction with the catalytic-site thiol group (Cys-25) of papain (EC 3.4.22.2) together with analogous kinetic data for the reactions of related time-dependent inhibitors, notably the …
The results of a study of the pH-dependence of the second-order rate constant (k) for its reaction with the catalytic-site thiol grou …
Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis.
Topham CM, Salih E, Frazao C, Kowlessur D, Overington JP, Thomas M, Brocklehurst SM, Patel M, Thomas EW, Brocklehurst K. Topham CM, et al. Among authors: brocklehurst sm, brocklehurst k. Biochem J. 1991 Nov 15;280 ( Pt 1)(Pt 1):79-92. doi: 10.1042/bj2800079. Biochem J. 1991. PMID: 1741760 Free PMC article.
Studies on streptococcal proteinase.
French H, Williams R, Salih E, Kowlessur D, Brocklehurst K. French H, et al. Among authors: brocklehurst k. Biochem Soc Trans. 1990 Aug;18(4):593-4. doi: 10.1042/bst0180593. Biochem Soc Trans. 1990. PMID: 2276454 No abstract available.
203 results