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AtGCS promoter-driven clustered regularly interspaced short palindromic repeats/Cas9 highly efficiently generates homozygous/biallelic mutations in the transformed roots by Agrobacterium rhizogenes-mediated transformation.
Liu S, Wang X, Li Q, Peng W, Zhang Z, Chu P, Guo S, Fan Y, Lyu S. Liu S, et al. Among authors: guo s. Front Plant Sci. 2022 Oct 18;13:952428. doi: 10.3389/fpls.2022.952428. eCollection 2022. Front Plant Sci. 2022. PMID: 36330262 Free PMC article.
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