Development of reverse genetics systems for bluetongue virus: recovery of infectious virus from synthetic RNA transcripts.
Boyce M, Celma CC, Roy P.
Boyce M, et al.
J Virol. 2008 Sep;82(17):8339-48. doi: 10.1128/JVI.00808-08. Epub 2008 Jun 18.
J Virol. 2008.
PMID: 18562540
Free PMC article.
Recovery of infectious BTV is possible by the transfection of permissive cells with the complete set of 10 purified viral mRNAs derived in vitro from transcribing cores (M. Boyce and P. Roy, J. Virol. 81:2179-2186, 2007). Here, we report that in vitro synthesized T7 …
Recovery of infectious BTV is possible by the transfection of permissive cells with the complete set of 10 purified viral mRNAs derived in v …