Tsukuda J - Search Results

1

Development and characterization of an automated imaging workflow to generate clonally-derived cell lines for therapeutic proteins.

Shaw D, Yim M, Tsukuda J, Joly JC, Lin A, Snedecor B, Laird MW, Lang SE. Shaw D, et al. Among authors: tsukuda j. Biotechnol Prog. 2018 May;34(3):584-592. doi: 10.1002/btpr.2561. Epub 2017 Oct 10. Biotechnol Prog. 2018. PMID: 28960825

2

A clone screening method using mRNA levels to determine specific productivity and product quality for monoclonal antibodies.

Lee CJ, Seth G, Tsukuda J, Hamilton RW. Lee CJ, et al. Among authors: tsukuda j. Biotechnol Bioeng. 2009 Mar 1;102(4):1107-18. doi: 10.1002/bit.22126. Biotechnol Bioeng. 2009. PMID: 18985612

3

Beating the odds: The poisson distribution of all input cells during limiting dilution grossly underestimates whether a cell line is clonally-derived or not.

Zhou Y, Shaw D, Lam C, Tsukuda J, Yim M, Tang D, Louie S, Laird MW, Snedecor B, Misaghi S. Zhou Y, et al. Among authors: tsukuda j. Biotechnol Prog. 2018 May;34(3):559-569. doi: 10.1002/btpr.2560. Epub 2017 Oct 9. Biotechnol Prog. 2018. PMID: 28941017

4

Probing the importance of clonality: Single cell subcloning of clonally derived CHO cell lines yields widely diverse clones differing in growth, productivity, and product quality.

Ko P, Misaghi S, Hu Z, Zhan D, Tsukuda J, Yim M, Sanford M, Shaw D, Shiratori M, Snedecor B, Laird M, Shen A. Ko P, et al. Among authors: tsukuda j. Biotechnol Prog. 2018 May;34(3):624-634. doi: 10.1002/btpr.2594. Epub 2017 Dec 27. Biotechnol Prog. 2018. PMID: 29226566

5

Concurrent transfection of randomized transgene configurations into targeted integration CHO host is an advantageous and cost-effective method for expression of complex molecules.

Dong E, Lam C, Tang D, Louie S, Yim M, Williams AJ, Sawyer W, Yip S, Carver J, AlBarakat A, Tsukuda J, Snedecor B, Misaghi S. Dong E, et al. Among authors: tsukuda j. Biotechnol J. 2021 Apr;16(4):e2000230. doi: 10.1002/biot.202000230. Epub 2020 Dec 30. Biotechnol J. 2021. PMID: 33259700

6

SPEED-MODE cell line development (CLD): Reducing Chinese hamster ovary (CHO) CLD timelines via earlier suspension adaptation and maximizing time spent in the exponential growth phase.

Ganapathy K, Lam C, Tsukuda J, Sargon A, Nava A, Harms P, Shen A, Barnard G, Misaghi S. Ganapathy K, et al. Among authors: tsukuda j. Biotechnol Prog. 2024 Sep-Oct;40(5):e3479. doi: 10.1002/btpr.3479. Epub 2024 May 8. Biotechnol Prog. 2024. PMID: 38716635

7

Engineering upper hinge improves stability and effector function of a human IgG1.

Yan B, Boyd D, Kaschak T, Tsukuda J, Shen A, Lin Y, Chung S, Gupta P, Kamath A, Wong A, Vernes JM, Meng GY, Totpal K, Schaefer G, Jiang G, Nogal B, Emery C, Vanderlaan M, Carter P, Harris R, Amanullah A. Yan B, et al. Among authors: tsukuda j. J Biol Chem. 2012 Feb 17;287(8):5891-7. doi: 10.1074/jbc.M111.311811. Epub 2011 Dec 27. J Biol Chem. 2012. PMID: 22203673 Free PMC article.

8

High titer expression of antibodies using linear expression cassettes for early-stage functional screening.

Wu S, Tsukuda J, Chiang N, Hao T, Chen Y, Hötzel I, Balasubramanian S, Nakamura G, Kelly RL. Wu S, et al. Among authors: tsukuda j. Protein Eng Des Sel. 2024 Jan 29;37:gzae012. doi: 10.1093/protein/gzae012. Protein Eng Des Sel. 2024. PMID: 39141844

9

Effectiveness of Extracorporeal Cardiopulmonary Resuscitation for Non-Shockable Out-of-Hospital Cardiac Arrest: An Analysis From the SOS-KANTO 2017 Study.

Yoshida T, Yoshida M, Masui Y, Naito T, Tsukuda J, Morisawa K, Fujitani S. Yoshida T, et al. Among authors: tsukuda j. Cureus. 2024 Sep 13;16(9):e69305. doi: 10.7759/cureus.69305. eCollection 2024 Sep. Cureus. 2024. PMID: 39398786 Free PMC article.

10

Comparison of the Incidence of Post-intensive Care Syndrome (PICS) Between Elderly and Non-elderly Patients: A Subgroup Analysis of the Japan-PICS Study.

Takita M, Kawakami D, Yoshida T, Tsukuda J, Fujitani S. Takita M, et al. Among authors: tsukuda j. Cureus. 2024 May 17;16(5):e60478. doi: 10.7759/cureus.60478. eCollection 2024 May. Cureus. 2024. PMID: 38882989 Free PMC article.

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