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Engineering the thermostability of β-glucuronidase from Penicillium purpurogenum Li-3 by loop transplant.
Feng X, Tang H, Han B, Zhang L, Lv B, Li C. Feng X, et al. Among authors: li c. Appl Microbiol Biotechnol. 2016 Dec;100(23):9955-9966. doi: 10.1007/s00253-016-7630-5. Epub 2016 Jun 21. Appl Microbiol Biotechnol. 2016. PMID: 27325137
In this study, we proposed a loop transplant strategy to improve the thermostability of Penicillium purpurogenum Li-3 beta-glucuronidase expressed in Escherichia coli (abbreviated to PGUS-E). Firstly, three unstable surface loops of PGUS-E to be replaced were identified wi …
In this study, we proposed a loop transplant strategy to improve the thermostability of Penicillium purpurogenum Li-3 beta-glucuronid …
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