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Cas9 gRNA engineering for genome editing, activation and repression.
Kiani S, Chavez A, Tuttle M, Hall RN, Chari R, Ter-Ovanesyan D, Qian J, Pruitt BW, Beal J, Vora S, Buchthal J, Kowal EJ, Ebrahimkhani MR, Collins JJ, Weiss R, Church G. Kiani S, et al. Nat Methods. 2015 Nov;12(11):1051-4. doi: 10.1038/nmeth.3580. Epub 2015 Sep 7. Nat Methods. 2015. PMID: 26344044 Free PMC article.
Comparison of Cas9 activators in multiple species.
Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJK, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G. Chavez A, et al. Among authors: kowal ejk. Nat Methods. 2016 Jul;13(7):563-567. doi: 10.1038/nmeth.3871. Epub 2016 May 23. Nat Methods. 2016. PMID: 27214048 Free PMC article.
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper.
Mateescu B, Kowal EJ, van Balkom BW, Bartel S, Bhattacharyya SN, Buzás EI, Buck AH, de Candia P, Chow FW, Das S, Driedonks TA, Fernández-Messina L, Haderk F, Hill AF, Jones JC, Van Keuren-Jensen KR, Lai CP, Lässer C, Liegro ID, Lunavat TR, Lorenowicz MJ, Maas SL, Mäger I, Mittelbrunn M, Momma S, Mukherjee K, Nawaz M, Pegtel DM, Pfaffl MW, Schiffelers RM, Tahara H, Théry C, Tosar JP, Wauben MH, Witwer KW, Nolte-'t Hoen EN. Mateescu B, et al. J Extracell Vesicles. 2017 Mar 7;6(1):1286095. doi: 10.1080/20013078.2017.1286095. eCollection 2017. J Extracell Vesicles. 2017. PMID: 28326170 Free PMC article.