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A Consensus Genome-scale Reconstruction of Chinese Hamster Ovary Cell Metabolism.
Hefzi H, Ang KS, Hanscho M, Bordbar A, Ruckerbauer D, Lakshmanan M, Orellana CA, Baycin-Hizal D, Huang Y, Ley D, Martinez VS, Kyriakopoulos S, Jiménez NE, Zielinski DC, Quek LE, Wulff T, Arnsdorf J, Li S, Lee JS, Paglia G, Loira N, Spahn PN, Pedersen LE, Gutierrez JM, King ZA, Lund AM, Nagarajan H, Thomas A, Abdel-Haleem AM, Zanghellini J, Kildegaard HF, Voldborg BG, Gerdtzen ZP, Betenbaugh MJ, Palsson BO, Andersen MR, Nielsen LK, Borth N, Lee DY, Lewis NE. Hefzi H, et al. Among authors: li s. Cell Syst. 2016 Nov 23;3(5):434-443.e8. doi: 10.1016/j.cels.2016.10.020. Cell Syst. 2016. PMID: 27883890 Free PMC article.
Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitations of protein secretion.
Gutierrez JM, Feizi A, Li S, Kallehauge TB, Hefzi H, Grav LM, Ley D, Baycin Hizal D, Betenbaugh MJ, Voldborg B, Faustrup Kildegaard H, Min Lee G, Palsson BO, Nielsen J, Lewis NE. Gutierrez JM, et al. Among authors: li s. Nat Commun. 2020 Jan 2;11(1):68. doi: 10.1038/s41467-019-13867-y. Nat Commun. 2020. PMID: 31896772 Free PMC article.
Awakening dormant glycosyltransferases in CHO cells with CRISPRa.
Karottki KJC, Hefzi H, Xiong K, Shamie I, Hansen AH, Li S, Pedersen LE, Li S, Lee JS, Lee GM, Kildegaard HF, Lewis NE. Karottki KJC, et al. Among authors: li s. Biotechnol Bioeng. 2020 Feb;117(2):593-598. doi: 10.1002/bit.27199. Epub 2019 Nov 12. Biotechnol Bioeng. 2020. PMID: 31631317 Free PMC article.
Combating viral contaminants in CHO cells by engineering innate immunity.
Chiang AWT, Li S, Kellman BP, Chattopadhyay G, Zhang Y, Kuo CC, Gutierrez JM, Ghazi F, Schmeisser H, Ménard P, Bjørn SP, Voldborg BG, Rosenberg AS, Puig M, Lewis NE. Chiang AWT, et al. Among authors: li s. Sci Rep. 2019 Jun 20;9(1):8827. doi: 10.1038/s41598-019-45126-x. Sci Rep. 2019. PMID: 31222165 Free PMC article.
Reduced apoptosis in Chinese hamster ovary cells via optimized CRISPR interference.
Xiong K, Marquart KF, la Cour Karottki KJ, Li S, Shamie I, Lee JS, Gerling S, Yeo NC, Chavez A, Lee GM, Lewis NE, Kildegaard HF. Xiong K, et al. Among authors: li s. Biotechnol Bioeng. 2019 Jul;116(7):1813-1819. doi: 10.1002/bit.26969. Epub 2019 Apr 2. Biotechnol Bioeng. 2019. PMID: 30883679 Free PMC article.
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