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Engineering the thermostability of β-glucuronidase from Penicillium purpurogenum Li-3 by loop transplant.
Feng X, Tang H, Han B, Zhang L, Lv B, Li C. Feng X, et al. Among authors: li c. Appl Microbiol Biotechnol. 2016 Dec;100(23):9955-9966. doi: 10.1007/s00253-016-7630-5. Epub 2016 Jun 21. Appl Microbiol Biotechnol. 2016. PMID: 27325137
In this study, we proposed a loop transplant strategy to improve the thermostability of Penicillium purpurogenum Li-3 beta-glucuronidase expressed in Escherichia coli (abbreviated to PGUS-E). Firstly, three unstable surface loops of PGUS-E to be replaced were identified wi …
In this study, we proposed a loop transplant strategy to improve the thermostability of Penicillium purpurogenum Li-3 beta-glucuronid …
Effects of low-shear modeled microgravity on the characterization of recombinant β-D-glucuronidase expressed in Pichia pastoris.
Qi F, Dai D, Liu Y, Kaleem I, Li C. Qi F, et al. Among authors: li c. Appl Biochem Biotechnol. 2011 Jan;163(1):162-72. doi: 10.1007/s12010-010-9025-x. Epub 2010 Jul 6. Appl Biochem Biotechnol. 2011. PMID: 20607443
EU095019) derived from Penicillium purpurogenum Li-3 encoding beta-D-glucuronidase (PGUS) was expressed in P. pastoris GS115 in two different environments of LSMMG and normal gravity (NG). Results manifested that both LSMMG and NG conditions had insignificant effects on te …
EU095019) derived from Penicillium purpurogenum Li-3 encoding beta-D-glucuronidase (PGUS) was expressed in P. pastoris GS115 in two d …
Effects of a non-conservative sequence on the properties of β-glucuronidase from Aspergillus terreus Li-20.
Liu Y, Huangfu J, Qi F, Kaleem I, E W, Li C. Liu Y, et al. Among authors: li c. PLoS One. 2012;7(2):e30998. doi: 10.1371/journal.pone.0030998. Epub 2012 Feb 7. PLoS One. 2012. PMID: 22347419 Free PMC article.
We cloned the beta-glucuronidase gene (AtGUS) from Aspergillus terreus Li-20 encoding 657 amino acids (aa), which can transform glycyrrhizin into glycyrrhetinic acid monoglucuronide (GAMG) and glycyrrhetinic acid (GA). Based on sequence alignment, the C-terminal non …
We cloned the beta-glucuronidase gene (AtGUS) from Aspergillus terreus Li-20 encoding 657 amino acids (aa), which can transform glycy …
90,040 results
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