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Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A.
Unzueta U, Vázquez F, Accardi G, Mendoza R, Toledo-Rubio V, Giuliani M, Sannino F, Parrilli E, Abasolo I, Schwartz S Jr, Tutino ML, Villaverde A, Corchero JL, Ferrer-Miralles N. Unzueta U, et al. Among authors: toledo rubio v. Appl Microbiol Biotechnol. 2015 Jul;99(14):5863-74. doi: 10.1007/s00253-014-6328-9. Epub 2015 Jan 24. Appl Microbiol Biotechnol. 2015. PMID: 25616525
Rehosting of bacterial chaperones for high-quality protein production.
Martínez-Alonso M, Toledo-Rubio V, Noad R, Unzueta U, Ferrer-Miralles N, Roy P, Villaverde A. Martínez-Alonso M, et al. Among authors: toledo rubio v. Appl Environ Microbiol. 2009 Dec;75(24):7850-4. doi: 10.1128/AEM.01532-09. Epub 2009 Oct 9. Appl Environ Microbiol. 2009. PMID: 19820142 Free PMC article.
Bacterial mimetics of endocrine secretory granules as immobilized in vivo depots for functional protein drugs.
Céspedes MV, Fernández Y, Unzueta U, Mendoza R, Seras-Franzoso J, Sánchez-Chardi A, Álamo P, Toledo-Rubio V, Ferrer-Miralles N, Vázquez E, Schwartz S, Abasolo I, Corchero JL, Mangues R, Villaverde A. Céspedes MV, et al. Among authors: toledo rubio v. Sci Rep. 2016 Oct 24;6:35765. doi: 10.1038/srep35765. Sci Rep. 2016. PMID: 27775083 Free PMC article.