Hytönen VP - Search Results

1

Kinetics of bioconjugate nanoparticle label binding in a sandwich-type immunoassay.

Näreoja T, Ebner A, Gruber HJ, Taskinen B, Kienberger F, Hänninen PE, Hytönen VP, Hinterdorfer P, Härmä H. Näreoja T, et al. Among authors: hytonen vp. Anal Bioanal Chem. 2014 Jan;406(2):493-503. doi: 10.1007/s00216-013-7474-0. Epub 2013 Nov 22. Anal Bioanal Chem. 2014. PMID: 24264621

2

Modification of the loops in the ligand-binding site turns avidin into a steroid-binding protein.

Riihimäki TA, Hiltunen S, Rangl M, Nordlund HR, Määttä JA, Ebner A, Hinterdorfer P, Kulomaa MS, Takkinen K, Hytönen VP. Riihimäki TA, et al. Among authors: hytonen vp. BMC Biotechnol. 2011 Jun 9;11:64. doi: 10.1186/1472-6750-11-64. BMC Biotechnol. 2011. PMID: 21658230 Free PMC article.

3

Reversible biofunctionalization of surfaces with a switchable mutant of avidin.

Pollheimer P, Taskinen B, Scherfler A, Gusenkov S, Creus M, Wiesauer P, Zauner D, Schöfberger W, Schwarzinger C, Ebner A, Tampé R, Stutz H, Hytönen VP, Gruber HJ. Pollheimer P, et al. Among authors: hytonen vp. Bioconjug Chem. 2013 Oct 16;24(10):1656-68. doi: 10.1021/bc400087e. Epub 2013 Sep 9. Bioconjug Chem. 2013. PMID: 23978112

4

Zebavidin--an avidin-like protein from zebrafish.

Taskinen B, Zmurko J, Ojanen M, Kukkurainen S, Parthiban M, Määttä JA, Leppiniemi J, Jänis J, Parikka M, Turpeinen H, Rämet M, Pesu M, Johnson MS, Kulomaa MS, Airenne TT, Hytönen VP. Taskinen B, et al. Among authors: hytonen vp. PLoS One. 2013 Oct 24;8(10):e77207. doi: 10.1371/journal.pone.0077207. eCollection 2013. PLoS One. 2013. PMID: 24204770 Free PMC article.

5

Investigating the binding behaviour of two avidin-based testosterone binders using molecular recognition force spectroscopy.

Rangl M, Leitner M, Riihimäki T, Lehtonen S, Hytönen VP, Gruber HJ, Kulomaa M, Hinterdorfer P, Ebner A. Rangl M, et al. Among authors: hytonen vp. J Mol Recognit. 2014 Feb;27(2):92-7. doi: 10.1002/jmr.2337. J Mol Recognit. 2014. PMID: 24436126

6

A novel chimeric avidin with increased thermal stability using DNA shuffling.

Taskinen B, Airenne TT, Jänis J, Rahikainen R, Johnson MS, Kulomaa MS, Hytönen VP. Taskinen B, et al. Among authors: hytonen vp. PLoS One. 2014 Mar 14;9(3):e92058. doi: 10.1371/journal.pone.0092058. eCollection 2014. PLoS One. 2014. PMID: 24632863 Free PMC article.

7

pH-dependent deformations of the energy landscape of avidin-like proteins investigated by single molecule force spectroscopy.

Köhler M, Karner A, Leitner M, Hytönen VP, Kulomaa M, Hinterdorfer P, Ebner A. Köhler M, et al. Among authors: hytonen vp. Molecules. 2014 Aug 18;19(8):12531-46. doi: 10.3390/molecules190812531. Molecules. 2014. PMID: 25153869 Free PMC article.

8

Switchavidin: reversible biotin-avidin-biotin bridges with high affinity and specificity.

Taskinen B, Zauner D, Lehtonen SI, Koskinen M, Thomson C, Kähkönen N, Kukkurainen S, Määttä JA, Ihalainen TO, Kulomaa MS, Gruber HJ, Hytönen VP. Taskinen B, et al. Among authors: hytonen vp. Bioconjug Chem. 2014 Dec 17;25(12):2233-43. doi: 10.1021/bc500462w. Epub 2014 Dec 1. Bioconjug Chem. 2014. PMID: 25405260

9

Efficient preparation of shuffled DNA libraries through recombination (Gateway) cloning.

Lehtonen SI, Taskinen B, Ojala E, Kukkurainen S, Rahikainen R, Riihimäki TA, Laitinen OH, Kulomaa MS, Hytönen VP. Lehtonen SI, et al. Among authors: hytonen vp. Protein Eng Des Sel. 2015 Jan;28(1):23-8. doi: 10.1093/protein/gzu050. Epub 2014 Dec 1. Protein Eng Des Sel. 2015. PMID: 25445152

10

Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein.

Oberbichler E, Wiesauer M, Schlögl E, Stangl J, Faschinger F, Knör G, Gruber HJ, Hytönen VP. Oberbichler E, et al. Among authors: hytonen vp. Methods Enzymol. 2020;633:1-20. doi: 10.1016/bs.mie.2019.10.028. Epub 2019 Nov 20. Methods Enzymol. 2020. PMID: 32046840

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