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Biochemical Methods to Analyze Wnt Protein Secretion.
Glaeser K, Boutros M, Gross JC. Glaeser K, et al. Among authors: gross jc. Methods Mol Biol. 2016;1481:17-28. doi: 10.1007/978-1-4939-6393-5_3. Methods Mol Biol. 2016. PMID: 27590148
Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches.
Welsh JA, Goberdhan DCI, O'Driscoll L, Buzas EI, Blenkiron C, Bussolati B, Cai H, Di Vizio D, Driedonks TAP, Erdbrügger U, Falcon-Perez JM, Fu QL, Hill AF, Lenassi M, Lim SK, Mahoney MG, Mohanty S, Möller A, Nieuwland R, Ochiya T, Sahoo S, Torrecilhas AC, Zheng L, Zijlstra A, Abuelreich S, Bagabas R, Bergese P, Bridges EM, Brucale M, Burger D, Carney RP, Cocucci E, Crescitelli R, Hanser E, Harris AL, Haughey NJ, Hendrix A, Ivanov AR, Jovanovic-Talisman T, Kruh-Garcia NA, Ku'ulei-Lyn Faustino V, Kyburz D, Lässer C, Lennon KM, Lötvall J, Maddox AL, Martens-Uzunova ES, Mizenko RR, Newman LA, Ridolfi A, Rohde E, Rojalin T, Rowland A, Saftics A, Sandau US, Saugstad JA, Shekari F, Swift S, Ter-Ovanesyan D, Tosar JP, Useckaite Z, Valle F, Varga Z, van der Pol E, van Herwijnen MJC, Wauben MHM, Wehman AM, Williams S, Zendrini A, Zimmerman AJ; MISEV Consortium; Théry C, Witwer KW. Welsh JA, et al. J Extracell Vesicles. 2024 Feb;13(2):e12404. doi: 10.1002/jev2.12404. J Extracell Vesicles. 2024. PMID: 38326288 Free PMC article.
Active Wnt proteins are secreted on exosomes.
Gross JC, Chaudhary V, Bartscherer K, Boutros M. Gross JC, et al. Nat Cell Biol. 2012 Oct;14(10):1036-45. doi: 10.1038/ncb2574. Epub 2012 Sep 16. Nat Cell Biol. 2012. PMID: 22983114
eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled.
Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, Karuna M P, Levkin P, Gross JC, Boutros M, Schulte G, Davidson G. Wesslowski J, et al. Among authors: gross jc. J Biol Chem. 2020 Jun 26;295(26):8759-8774. doi: 10.1074/jbc.RA120.012892. Epub 2020 May 7. J Biol Chem. 2020. PMID: 32381507 Free PMC article.
50 results