de Gier JW - Search Results

1

Optimizing E. coli-based membrane protein production using Lemo21(DE3) and GFP-fusions.

Hjelm A, Schlegel S, Baumgarten T, Klepsch M, Wickström D, Drew D, de Gier JW. Hjelm A, et al. Among authors: de gier jw. Methods Mol Biol. 2013;1033:381-400. doi: 10.1007/978-1-62703-487-6_24. Methods Mol Biol. 2013. PMID: 23996190

2

Optimization of membrane protein overexpression and purification using GFP fusions.

Drew D, Lerch M, Kunji E, Slotboom DJ, de Gier JW. Drew D, et al. Among authors: de gier jw. Nat Methods. 2006 Apr;3(4):303-13. doi: 10.1038/nmeth0406-303. Nat Methods. 2006. PMID: 16554836 No abstract available.

3

Rationalizing membrane protein overexpression.

Wagner S, Bader ML, Drew D, de Gier JW. Wagner S, et al. Among authors: de gier jw. Trends Biotechnol. 2006 Aug;24(8):364-71. doi: 10.1016/j.tibtech.2006.06.008. Epub 2006 Jul 3. Trends Biotechnol. 2006. PMID: 16820235 Review.

4

Tuning Escherichia coli for membrane protein overexpression.

Wagner S, Klepsch MM, Schlegel S, Appel A, Draheim R, Tarry M, Högbom M, van Wijk KJ, Slotboom DJ, Persson JO, de Gier JW. Wagner S, et al. Among authors: de gier jw. Proc Natl Acad Sci U S A. 2008 Sep 23;105(38):14371-6. doi: 10.1073/pnas.0804090105. Epub 2008 Sep 16. Proc Natl Acad Sci U S A. 2008. PMID: 18796603 Free PMC article.

5

Comparative analysis of cytoplasmic membrane proteomes of Escherichia coli using 2D blue native/SDS-PAGE.

Schlegel S, Klepsch M, Wickström D, Wagner S, de Gier JW. Schlegel S, et al. Among authors: de gier jw. Methods Mol Biol. 2010;619:257-69. doi: 10.1007/978-1-60327-412-8_15. Methods Mol Biol. 2010. PMID: 20419415

6

Revolutionizing membrane protein overexpression in bacteria.

Schlegel S, Klepsch M, Gialama D, Wickström D, Slotboom DJ, de Gier JW. Schlegel S, et al. Among authors: de gier jw. Microb Biotechnol. 2010 Jul;3(4):403-11. doi: 10.1111/j.1751-7915.2009.00148.x. Epub 2009 Sep 4. Microb Biotechnol. 2010. PMID: 21255339 Free PMC article. Review.

7

Optimizing membrane protein overexpression in the Escherichia coli strain Lemo21(DE3).

Schlegel S, Löfblom J, Lee C, Hjelm A, Klepsch M, Strous M, Drew D, Slotboom DJ, de Gier JW. Schlegel S, et al. Among authors: de gier jw. J Mol Biol. 2012 Nov 2;423(4):648-59. doi: 10.1016/j.jmb.2012.07.019. Epub 2012 Jul 31. J Mol Biol. 2012. PMID: 22858868

8

Optimizing heterologous protein production in the periplasm of E. coli by regulating gene expression levels.

Schlegel S, Rujas E, Ytterberg AJ, Zubarev RA, Luirink J, de Gier JW. Schlegel S, et al. Among authors: de gier jw. Microb Cell Fact. 2013 Mar 12;12:24. doi: 10.1186/1475-2859-12-24. Microb Cell Fact. 2013. PMID: 23497240 Free PMC article.

9

Bacterial-based membrane protein production.

Schlegel S, Hjelm A, Baumgarten T, Vikström D, de Gier JW. Schlegel S, et al. Among authors: de gier jw. Biochim Biophys Acta. 2014 Aug;1843(8):1739-49. doi: 10.1016/j.bbamcr.2013.10.023. Epub 2013 Nov 5. Biochim Biophys Acta. 2014. PMID: 24200679 Free article. Review.

10

MemStar: a one-shot Escherichia coli-based approach for high-level bacterial membrane protein production.

Lee C, Kang HJ, Hjelm A, Qureshi AA, Nji E, Choudhury H, Beis K, de Gier JW, Drew D. Lee C, et al. Among authors: de gier jw. FEBS Lett. 2014 Oct 16;588(20):3761-9. doi: 10.1016/j.febslet.2014.08.025. Epub 2014 Aug 28. FEBS Lett. 2014. PMID: 25176409 Free article.

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