PolyA RT-PCR-based quantification of microRNA by using universal TaqMan probe.
Luo X, Zhang J, Wang H, Du Y, Yang L, Zheng F, Ma D.
Luo X, et al. Among authors: zhang j.
Biotechnol Lett. 2012 Apr;34(4):627-33. doi: 10.1007/s10529-011-0813-3. Epub 2011 Dec 9.
Biotechnol Lett. 2012.
PMID: 22160364
Based on a PolyA RT-PCR method we have described (J Zhang et al. Biochem Biophys Res Commun 2008 377:136-140), a modified miRNA quantification method was developed and validated using a universal TaqMan probe complementary to the reverse transcript primer. ...
Based on a PolyA RT-PCR method we have described (J Zhang et al. Biochem Biophys Res Commun 2008 377:136-140), a modified miRN …