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Large-scale purification of human BACE expressed in mammalian cells and removal of the prosegment with HIV-1 protease to improve crystal diffraction.
Emmons TL, Shuck ME, Babcock MS, Holloway JS, Leone JW, Durbin JD, Paddock DJ, Prince DB, Heinrikson RL, Fischer HD, Bienkowski MJ, Benson TE, Tomasselli AG. Emmons TL, et al. Among authors: leone jw. Protein Pept Lett. 2008;15(2):119-30. doi: 10.2174/092986608783489599. Protein Pept Lett. 2008. PMID: 18289104
Expression, purification, characterization and crystallization of non- and phosphorylated states of JAK2 and JAK3 kinase domain.
Hall T, Emmons TL, Chrencik JE, Gormley JA, Weinberg RA, Leone JW, Hirsch JL, Saabye MJ, Schindler JF, Day JE, Williams JM, Kiefer JR, Lightle SA, Harris MS, Guru S, Fischer HD, Tomasselli AG. Hall T, et al. Among authors: leone jw. Protein Expr Purif. 2010 Jan;69(1):54-63. doi: 10.1016/j.pep.2009.09.011. Epub 2009 Sep 23. Protein Expr Purif. 2010. PMID: 19781647
Expression, purification, and characterization of TYK-2 kinase domain, a member of the Janus kinase family.
Korniski B, Wittwer AJ, Emmons TL, Hall T, Brown S, Wrightstone AD, Hirsch JL, Gormley JA, Weinberg RA, Leone JW, Day JE, Chrencik JE, Sommers CD, Fischer HD, Tomasselli AG. Korniski B, et al. Among authors: leone jw. Biochem Biophys Res Commun. 2010 May 28;396(2):543-8. doi: 10.1016/j.bbrc.2010.04.141. Epub 2010 May 8. Biochem Biophys Res Commun. 2010. PMID: 20435017
Employing a superior BACE1 cleavage sequence to probe cellular APP processing.
Tomasselli AG, Qahwash I, Emmons TL, Lu Y, Leone JW, Lull JM, Fok KF, Bannow CA, Smith CW, Bienkowski MJ, Heinrikson RL, Yan R. Tomasselli AG, et al. Among authors: leone jw. J Neurochem. 2003 Mar;84(5):1006-17. doi: 10.1046/j.1471-4159.2003.01597.x. J Neurochem. 2003. PMID: 12603825 Free article.
High resolution crystal structure of the catalytic domain of ADAMTS-5 (aggrecanase-2).
Shieh HS, Mathis KJ, Williams JM, Hills RL, Wiese JF, Benson TE, Kiefer JR, Marino MH, Carroll JN, Leone JW, Malfait AM, Arner EC, Tortorella MD, Tomasselli A. Shieh HS, et al. Among authors: leone jw. J Biol Chem. 2008 Jan 18;283(3):1501-1507. doi: 10.1074/jbc.M705879200. Epub 2007 Nov 8. J Biol Chem. 2008. PMID: 17991750 Free article.
40 results