Iwai H - Search Results

1

Protein ligation: applications in NMR studies of proteins.

Iwai H, Züger S. Iwai H, et al. Biotechnol Genet Eng Rev. 2007;24:129-45. doi: 10.1080/02648725.2007.10648096. Biotechnol Genet Eng Rev. 2007. PMID: 18059630 Review. No abstract available.

2

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

Züger S, Iwai H. Züger S, et al. Among authors: iwai h. Nat Biotechnol. 2005 Jun;23(6):736-40. doi: 10.1038/nbt1097. Epub 2005 May 22. Nat Biotechnol. 2005. PMID: 15908942

3

Highly efficient protein trans-splicing by a naturally split DnaE intein from Nostoc punctiforme.

Iwai H, Züger S, Jin J, Tam PH. Iwai H, et al. FEBS Lett. 2006 Mar 20;580(7):1853-8. doi: 10.1016/j.febslet.2006.02.045. Epub 2006 Feb 24. FEBS Lett. 2006. PMID: 16516207 Free article.

4

Use of biosynthetic fractional 13C-labeling for backbone NMR assignment of proteins.

Iwai H, Fiaux J. Iwai H, et al. J Biomol NMR. 2007 Mar;37(3):187-93. doi: 10.1007/s10858-006-9124-8. Epub 2007 Jan 13. J Biomol NMR. 2007. PMID: 17221288

5

Segmental isotopic labelling of a multidomain protein by protein ligation by protein trans-splicing.

Muona M, Aranko AS, Iwai H. Muona M, et al. Among authors: iwai h. Chembiochem. 2008 Dec 15;9(18):2958-61. doi: 10.1002/cbic.200800604. Chembiochem. 2008. PMID: 19031436 No abstract available.

6

In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins.

Aranko AS, Züger S, Buchinger E, Iwaï H. Aranko AS, et al. Among authors: iwai h. PLoS One. 2009;4(4):e5185. doi: 10.1371/journal.pone.0005185. Epub 2009 Apr 13. PLoS One. 2009. PMID: 19365564 Free PMC article.

7

NMR resonance assignment of DnaE intein from Nostoc punctiforme.

Heinämäki K, Oeemig JS, Pääkkönen K, Djupsjöbacka J, Iwaï H. Heinämäki K, et al. Among authors: iwai h. Biomol NMR Assign. 2009 Jun;3(1):41-3. doi: 10.1007/s12104-008-9137-1. Epub 2009 Jan 13. Biomol NMR Assign. 2009. PMID: 19636943

8

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro.

Muona M, Aranko AS, Raulinaitis V, Iwaï H. Muona M, et al. Among authors: iwai h. Nat Protoc. 2010 Mar;5(3):574-87. doi: 10.1038/nprot.2009.240. Epub 2010 Mar 4. Nat Protoc. 2010. PMID: 20203672

9

Structural basis for protein trans-splicing by a bacterial intein-like domain--protein ligation without nucleophilic side chains.

Aranko AS, Oeemig JS, Iwaï H. Aranko AS, et al. Among authors: iwai h. FEBS J. 2013 Jul;280(14):3256-69. doi: 10.1111/febs.12307. Epub 2013 May 28. FEBS J. 2013. PMID: 23621571 Free article.

10

Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation.

Mikula KM, Krumwiede L, Plückthun A, Iwaï H. Mikula KM, et al. Among authors: iwai h. J Biomol NMR. 2018 Aug;71(4):225-235. doi: 10.1007/s10858-018-0175-4. Epub 2018 Mar 13. J Biomol NMR. 2018. PMID: 29536230

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