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Combinatorial saturation mutagenesis of the Myceliophthora thermophila laccase T2 mutant: the connection between the C-terminal plug and the conserved (509)VSG(511) tripeptide.
Zumárraga M, Vaz Domínguez C, Camarero S, Shleev S, Polaina J, Martínez-Arias A, Ferrer M, De Lacey AL, Fernández VM, Ballesteros A, Plou FJ, Alcalde M. Zumárraga M, et al. Among authors: polaina j. Comb Chem High Throughput Screen. 2008 Dec;11(10):807-16. doi: 10.2174/138620708786734235. Comb Chem High Throughput Screen. 2008. PMID: 19075602
Conversion of a carboxylesterase into a triacylglycerol lipase by a random mutation.
Reyes-Duarte D, Polaina J, López-Cortés N, Alcalde M, Plou FJ, Elborough K, Ballesteros A, Timmis KN, Golyshin PN, Ferrer M. Reyes-Duarte D, et al. Among authors: polaina j. Angew Chem Int Ed Engl. 2005 Nov 25;44(46):7553-7. doi: 10.1002/anie.200502461. Angew Chem Int Ed Engl. 2005. PMID: 16254934 No abstract available.
Novel polyphenol oxidase mined from a metagenome expression library of bovine rumen: biochemical properties, structural analysis, and phylogenetic relationships.
Beloqui A, Pita M, Polaina J, Martínez-Arias A, Golyshina OV, Zumárraga M, Yakimov MM, García-Arellano H, Alcalde M, Fernández VM, Elborough K, Andreu JM, Ballesteros A, Plou FJ, Timmis KN, Ferrer M, Golyshin PN. Beloqui A, et al. Among authors: polaina j. J Biol Chem. 2006 Aug 11;281(32):22933-42. doi: 10.1074/jbc.M600577200. Epub 2006 Jun 1. J Biol Chem. 2006. PMID: 16740638 Free article.
Enzymatic Synthesis of a Novel Pterostilbene α-Glucoside by the Combination of Cyclodextrin Glucanotransferase and Amyloglucosidase.
González-Alfonso JL, Rodrigo-Frutos D, Belmonte-Reche E, Peñalver P, Poveda A, Jiménez-Barbero J, Ballesteros AO, Hirose Y, Polaina J, Morales JC, Fernández-Lobato M, Plou FJ. González-Alfonso JL, et al. Among authors: polaina j. Molecules. 2018 May 25;23(6):1271. doi: 10.3390/molecules23061271. Molecules. 2018. PMID: 29799509 Free PMC article.
Catalytic role of conserved HQGE motif in the CE6 carbohydrate esterase family.
López-Cortés N, Reyes-Duarte D, Beloqui A, Polaina J, Ghazi I, Golyshina OV, Ballesteros A, Golyshin PN, Ferrer M. López-Cortés N, et al. Among authors: polaina j. FEBS Lett. 2007 Oct 2;581(24):4657-62. doi: 10.1016/j.febslet.2007.08.060. Epub 2007 Sep 4. FEBS Lett. 2007. PMID: 17826771 Free article.
83 results