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Process development of a human recombinant diabody expressed in E. coli: engagement of CD99-induced apoptosis for target therapy in Ewing's sarcoma.
Moricoli D, Carbonella DC, Dominici S, Fiori V, Balducci MC, Guerzoni C, Manara MC, Pasello M, Laguardia ME, Cianfriglia M, Scotlandi K, Magnani M. Moricoli D, et al. Among authors: carbonella dc. Appl Microbiol Biotechnol. 2016 May;100(9):3949-63. doi: 10.1007/s00253-015-7226-5. Epub 2015 Dec 21. Appl Microbiol Biotechnol. 2016. PMID: 26685854
Isolation of a new human scFv antibody recognizing a cell surface binding site to CEACAM1. Large yield production, purification and characterization in E. coli expression system.
Moricoli D, Laguardia ME, Carbonella DC, Balducci MC, Dominici S, Fiori V, Serafini G, Flego M, Cianfriglia M, Magnani M. Moricoli D, et al. Among authors: carbonella dc. Protein Expr Purif. 2014 Jan;93:38-45. doi: 10.1016/j.pep.2013.10.009. Epub 2013 Oct 30. Protein Expr Purif. 2014. PMID: 24184403
Blocking monocyte transmigration in in vitro system by a human antibody scFv anti-CD99. Efficient large scale purification from periplasmic inclusion bodies in E. coli expression system.
Moricoli D, Muller WA, Carbonella DC, Balducci MC, Dominici S, Watson R, Fiori V, Weber E, Cianfriglia M, Scotlandi K, Magnani M. Moricoli D, et al. Among authors: carbonella dc. J Immunol Methods. 2014 Jun;408:35-45. doi: 10.1016/j.jim.2014.04.012. Epub 2014 May 4. J Immunol Methods. 2014. PMID: 24798881 Free PMC article.