Multifunctional role of Tyr 108 in the catalytic mechanism of human glutathione transferase P1-1. Crystallographic and kinetic studies on the Y108F mutant enzyme.
Lo Bello M, Oakley AJ, Battistoni A, Mazzetti AP, Nuccetelli M, Mazzarese G, Rossjohn J, Parker MW, Ricci G.
Lo Bello M, et al. Among authors: oakley aj.
Biochemistry. 1997 May 20;36(20):6207-17. doi: 10.1021/bi962813z.
Biochemistry. 1997.
PMID: 9166793
In the presence of ethacrynic acid, the enzyme follows a rapid equilibrium random bi-bi mechanism with a rate-limiting step which occurs after the addition of the substrates and before the release of products. ...Crystallographic data are compatible with this conclu …
In the presence of ethacrynic acid, the enzyme follows a rapid equilibrium random bi-bi mechanism with a rate-limiting step wh …