Binding of O(2) and its reduction are both retarded by replacement of valine 279 by isoleucine in cytochrome c oxidase from Paracoccus denitrificans.
Riistama S, Puustinen A, Verkhovsky MI, Morgan JE, Wikström M.
Riistama S, et al. Among authors: puustinen a.
Biochemistry. 2000 May 30;39(21):6365-72. doi: 10.1021/bi000123w.
Biochemistry. 2000.
PMID: 10828950
The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) reduction. Changing a conserved valine near this center in cytochrome bo(3) of Escherichia coli to isoleucine caused a significant …
The crystal structure of the heme-copper oxidases suggested a putative channel of oxygen entry into the heme-copper site of O(2) redu …