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Detection of low-frequency antigen-specific IL-10-producing CD4(+) T cells via ELISPOT in PBMC: cognate vs. nonspecific production of the cytokine.
J Immunol Methods. 2003 Aug;279(1-2):111-21. doi: 10.1016/s0022-1759(03)00240-0.
J Immunol Methods. 2003.
PMID: 12969552
CD4+ and CD8+ cells in cryopreserved human PBMC maintain full functionality in cytokine ELISPOT assays.
Kreher CR, Dittrich MT, Guerkov R, Boehm BO, Tary-Lehmann M.
Kreher CR, et al.
J Immunol Methods. 2003 Jul;278(1-2):79-93. doi: 10.1016/s0022-1759(03)00226-6.
J Immunol Methods. 2003.
PMID: 12957398
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CD28 costimulation enhances the sensitivity of the ELISPOT assay for detection of antigen-specific memory effector CD4 and CD8 cell populations in human diseases.
Ott PA, Berner BR, Herzog BA, Guerkov R, Yonkers NL, Durinovic-Bello I, Tary-Lehmann M, Lehmann PV, Anthony DD.
Ott PA, et al.
J Immunol Methods. 2004 Feb 15;285(2):223-35. doi: 10.1016/j.jim.2003.12.007.
J Immunol Methods. 2004.
PMID: 14980436
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T cells recognize multiple GAD65 and proinsulin epitopes in human type 1 diabetes, suggesting determinant spreading.
Ott PA, Dittrich MT, Herzog BA, Guerkov R, Gottlieb PA, Putnam AL, Durinovic-Bello I, Boehm BO, Tary-Lehmann M, Lehmann PV.
Ott PA, et al.
J Clin Immunol. 2004 Jul;24(4):327-39. doi: 10.1023/B:JOCI.0000029120.77824.41.
J Clin Immunol. 2004.
PMID: 15163889
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