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203 results

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Improvement in hydrolytic antibody activity by change in haptenic structure from phosphate to phosphonate with retention of a common leaving-group determinant: evidence for the 'flexibility' hypothesis.
Gul S, Sonkaria S, Pinitglang S, Florez-Alvarez J, Hussain S, Thomas EW, Ostler EL, Gallacher G, Resmini M, Brocklehurst K. Gul S, et al. Among authors: brocklehurst k. Biochem J. 2003 Dec 15;376(Pt 3):813-21. doi: 10.1042/BJ20030716. Biochem J. 2003. PMID: 12946271 Free PMC article.
Variation in the pH-dependent pre-steady-state and steady-state kinetic characteristics of cysteine-proteinase mechanism: evidence for electrostatic modulation of catalytic-site function by the neighbouring carboxylate anion.
Hussain S, Pinitglang S, Bailey TS, Reid JD, Noble MA, Resmini M, Thomas EW, Greaves RB, Verma CS, Brocklehurst K. Hussain S, et al. Among authors: brocklehurst k. Biochem J. 2003 Jun 15;372(Pt 3):735-46. doi: 10.1042/BJ20030177. Biochem J. 2003. PMID: 12643810 Free PMC article.
Evidence for 'lock and key' character in an anti-phosphonate hydrolytic antibody catalytic site augmented by non-reaction centre recognition: variation in substrate selectivity between an anti-phosphonate antibody, an anti-phosphate antibody and two hydrolytic enzymes.
Sonkaria S, Boucher G, Flórez-Olvarez J, Said B, Hussain S, Ostler EL, Gul S, Thomas EW, Resmini M, Gallacher G, Brocklehurst K. Sonkaria S, et al. Among authors: brocklehurst k. Biochem J. 2004 Jul 1;381(Pt 1):125-30. doi: 10.1042/BJ20031966. Biochem J. 2004. PMID: 15053743 Free PMC article.
Polyclonal antibody-catalysed amide hydrolysis.
Gallacher G, Searcey M, Jackson CS, Brocklehurst K. Gallacher G, et al. Among authors: brocklehurst k. Biochem J. 1992 Jun 15;284 ( Pt 3)(Pt 3):675-80. doi: 10.1042/bj2840675. Biochem J. 1992. PMID: 1622388 Free PMC article.
A polyclonal antibody preparation (PCA 270-29) was elicited in a multigeneration cross-bred sheep (no. 270) and isolated 29 weeks into the immunization schedule by procedures described previously for PCA 270-22 [Gallacher, Jackson, Searcey, Badman, Goel, Topham, Mellor & B …
A polyclonal antibody preparation (PCA 270-29) was elicited in a multigeneration cross-bred sheep (no. 270) and isolated 29 weeks into the i …
Ionization characteristics of the Cys-25/His-159 interactive system and of the modulatory group of papain: resolution of ambiguity by electronic perturbation of the quasi-2-mercaptopyridine leaving group in a new pyrimidyl disulphide reactivity probe.
Mellor GW, Thomas EW, Topham CM, Brocklehurst K. Mellor GW, et al. Among authors: brocklehurst k. Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):289-96. doi: 10.1042/bj2900289. Biochem J. 1993. PMID: 8439297 Free PMC article.
Characterization of the hydrolytic activity of a polyclonal catalytic antibody preparation by pH-dependence and chemical modification studies: evidence for the involvement of Tyr and Arg side chains as hydrogen-bond donors.
Resmini M, Vigna R, Simms C, Barber NJ, Hagi-Pavli EP, Watts AB, Verma C, Gallacher G, Brocklehurst K. Resmini M, et al. Among authors: brocklehurst k. Biochem J. 1997 Aug 15;326 ( Pt 1)(Pt 1):279-87. doi: 10.1042/bj3260279. Biochem J. 1997. PMID: 9337880 Free PMC article.
203 results