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alpha Arg-237 in Methylophilus methylotrophus (sp. W3A1) electron-transferring flavoprotein affords approximately 200-millivolt stabilization of the FAD anionic semiquinone and a kinetic block on full reduction to the dihydroquinone.
Talfournier F, Munro AW, Basran J, Sutcliffe MJ, Daff S, Chapman SK, Scrutton NS. Talfournier F, et al. J Biol Chem. 2001 Jun 8;276(23):20190-6. doi: 10.1074/jbc.M010853200. Epub 2001 Apr 2. J Biol Chem. 2001. PMID: 11285259 Free article.
CRDSAT Generated by pCARGHO: A New Efficient Lectin-Based Affinity Tag Method for Safe, Simple, and Low-Cost Protein Purification.
Kriznik A, Yéléhé-Okouma M, Lec JC, Groshenry G, Le Cordier H, Charron C, Quinternet M, Mazon H, Talfournier F, Boschi-Muller S, Jouzeau JY, Reboul P. Kriznik A, et al. Among authors: talfournier f. Biotechnol J. 2019 Apr;14(4):e1800214. doi: 10.1002/biot.201800214. Epub 2018 Oct 18. Biotechnol J. 2019. PMID: 30298550 Free article.
Expression, purification, crystallization and preliminary X-ray diffraction data of methylmalonate-semialdehyde dehydrogenase from Bacillus subtilis.
Dubourg H, Stines-Chaumeil C, Didierjean C, Talfournier F, Rahuel-Clermont S, Branlant G, Aubry A. Dubourg H, et al. Among authors: talfournier f. Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1435-7. doi: 10.1107/S0907444904012533. Epub 2004 Jul 21. Acta Crystallogr D Biol Crystallogr. 2004. PMID: 15272169
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