Essential lysine residues in the N-terminal and the C-terminal domain of human adenylate kinase interact with adenine nucleotides as found by site-directed random mutagenesis.
Ayabe T, Takenaka H, Takenaka O, Sumida M, Maruyama H, Onitsuka T, Shibata K, Uesugi S, Hamada M.
Ayabe T, et al.
Biochemistry. 1997 Apr 1;36(13):4027-33. doi: 10.1021/bi961796a.
Biochemistry. 1997.
PMID: 9092833
To elucidate the minimum requirement of amino acid residues for the active center in human adenylate kinase (hAK1), we carried out random site-directed mutagenesis of key lysine residues (K9, K21, K27, K31, K63, K131, and K194), which were conserved in mammalian AK1 species, with …
To elucidate the minimum requirement of amino acid residues for the active center in human adenylate kinase (hAK1), we carried out random si …