One-step purification and biochemical characterization of recombinant pancreatic lipases expressed in insect cells

Methods Mol Biol. 1999:109:187-202. doi: 10.1385/1-59259-581-2:187.
No abstract available

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cell Line
  • Chromatography, Liquid / instrumentation
  • Chromatography, Liquid / methods
  • DNA, Complementary / genetics
  • Genetic Vectors / genetics*
  • Humans
  • Kinetics
  • Lipase / biosynthesis
  • Lipase / genetics
  • Lipase / isolation & purification*
  • Moths
  • Nucleopolyhedroviruses / genetics*
  • Pancreas / enzymology*
  • Placenta / chemistry
  • RNA, Messenger / genetics
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / isolation & purification
  • Rodentia
  • Spodoptera / chemistry
  • Spodoptera / cytology
  • Triglycerides / metabolism

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • Triglycerides
  • Lipase
  • tributyrin