Structural characteristics of low-molecular-mass displacers for cation-exchange chromatography. II. Role of the stationary phase

J Chromatogr A. 1998 Dec 11;827(2):295-310. doi: 10.1016/s0021-9673(98)00805-x.

Abstract

The relative efficacy of a variety of low-molecular-mass displacers was examined on three different stationary phase materials. Several homologous series of displacer molecules were evaluated on these ion-exchange resins using a displacer ranking plot based on the steric mass action model. The results demonstrate that while aromaticity and hydrophobicity can play a significant role in the affinity of displacer molecules on polymethacrylate based and hydrophilized polystyrene-divinylbenzene based materials, this effect is much less pronounced on an agarose based resin. The work presented in this paper demonstrates that different structural features of low-molecular-mass displacers can dominate their affinity on various stationary phase materials employed and provides rules of thumb for the design of high affinity, low-molecular-mass displacers for a variety of commercial cation-exchange materials.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cation Exchange Resins
  • Chromatography, Ion Exchange / methods*
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Spectrophotometry, Ultraviolet

Substances

  • Cation Exchange Resins