Comparative study of serum and synovial fluid interleukin-11 levels in patients with various arthritides

Clin Biochem. 1998 Nov;31(8):673-9. doi: 10.1016/s0009-9120(98)00062-9.

Abstract

Objective: To determine the levels of serum and synovial fluid (SF) interleukin (IL)-11 in patients with various arthritides and estimate the contribution of IL-11 to acute phase response (APR).

Design and methods: Serum and SF IL-11 were measured by ELISA in patients with rheumatoid arthritis (RA, n = 31), seronegative spondyloarthritis (SSA, n = 23), gout (GT, n = 14) and osteoarthritis (OA, n = 20) and were correlated with ESR and acute phase proteins as well as with cytokines IL-1 alpha, IL-1 beta, IL-6, and TNF alpha.

Results: IL-11 was detected in both serum and SF in each group, with IL-11 being statistically higher in SF than serum in all groups, suggesting reduced catabolism or increased synthesis of IL-11 intra-articularly. Median SF IL-11 levels were higher in OA patients than in other groups and in the treated than in the untreated RA subgroup. Moreover, serum and SF IL-11 were correlated significantly with each other, and moderately with the other cytokines examined in RA, SSA, and GT, but not in OA patients, while a significant negative correlation was found with a few of the inflammatory markers examined in each group.

Conclusions: Our findings provide evidence of extensive intra-articular expression of IL-11 in arthritides, especially in OA and treated RA patients, suggesting a protective role for IL-11 in joints, probably through the induction of tissue inhibitor of metalloproteinases.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute-Phase Proteins / metabolism*
  • Acute-Phase Reaction / metabolism
  • Arthritis / metabolism*
  • Arthritis, Rheumatoid / physiopathology
  • Biomarkers
  • C-Reactive Protein / metabolism
  • Ceruloplasmin / metabolism
  • Cytokines / metabolism
  • Enzyme-Linked Immunosorbent Assay
  • Ferritins / metabolism
  • Fibrinogen / metabolism
  • Gout / physiopathology
  • Haptoglobins / metabolism
  • Humans
  • Immunodiffusion
  • Interleukin-1 / metabolism
  • Interleukin-11 / blood*
  • Interleukin-11 / metabolism
  • Interleukin-6 / metabolism
  • Nephelometry and Turbidimetry
  • Orosomucoid / metabolism
  • Osteoarthritis / physiopathology
  • Prealbumin / metabolism
  • Prospective Studies
  • Statistics as Topic
  • Statistics, Nonparametric
  • Synovial Fluid / metabolism*
  • Transferrin / metabolism
  • Tumor Necrosis Factor-alpha / metabolism
  • alpha 1-Antitrypsin / metabolism
  • alpha-Macroglobulins / metabolism
  • beta 2-Microglobulin / metabolism

Substances

  • Acute-Phase Proteins
  • Biomarkers
  • Cytokines
  • Haptoglobins
  • Interleukin-1
  • Interleukin-11
  • Interleukin-6
  • Orosomucoid
  • Prealbumin
  • Transferrin
  • Tumor Necrosis Factor-alpha
  • alpha 1-Antitrypsin
  • alpha-Macroglobulins
  • beta 2-Microglobulin
  • Fibrinogen
  • C-Reactive Protein
  • Ferritins
  • Ceruloplasmin