Substrate specificity of human prolyl-4-hydroxylase

M Tandon; M Wu; T P Begley; J Myllyharju; A Pirskanen; K Kivirikko

doi:10.1016/s0960-894x(98)00183-8

Substrate specificity of human prolyl-4-hydroxylase

Bioorg Med Chem Lett. 1998 May 19;8(10):1139-44. doi: 10.1016/s0960-894x(98)00183-8.

Authors

M Tandon¹, M Wu, T P Begley, J Myllyharju, A Pirskanen, K Kivirikko

Affiliation

¹ Department of Chemistry, Cornell University, Ithaca, New York 14853, USA.

PMID: 9871723
DOI: 10.1016/s0960-894x(98)00183-8

Abstract

Proline analogs (3-F, 3-Cl, 3-Br, 3,3-cyclopropyl, 3,3-methylene, 3-Me, and 4-Me) were synthesized, incorporated into CbzGlyPheXGlyOEt, and tested as substrate analogues/mechanistic probes for the human prolyl-4-hydroxylase catalyzed hydroxylation reaction. With the exception of the 3-fluoroproline containing peptide, none of these peptides were substrates for the enzyme.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Humans
Hydroxylation
Indicators and Reagents
Molecular Structure
Procollagen-Proline Dioxygenase / metabolism*
Proline / analogs & derivatives*
Proline / chemical synthesis
Proline / chemistry
Proline / metabolism*
Structure-Activity Relationship
Substrate Specificity

Substances

Indicators and Reagents
Proline
Procollagen-Proline Dioxygenase