Synthesis of 14 C-radiolabeled oligonucleotides with a novel phosphoramidite reagent

Bioorg Med Chem Lett. 1998 May 5;8(9):1123-6. doi: 10.1016/s0960-894x(98)00028-6.

Abstract

A novel solid-phase synthesis of 5'-radiolabeled oligonucleotides is described. The labeling reaction is carried out by the phosphoramidite method with the aid of [4,6-di-14C]-5'-dimethoxytritylthymidine building block 1. The feasibility of the method is demonstrated by preparation of 3'-phosphorylated dodecathymidylate phosphorothioate containing radiolabeled nucleoside at the 5'-terminus.

MeSH terms

  • Carbon Radioisotopes
  • Indicators and Reagents
  • Isotope Labeling / methods
  • Models, Molecular
  • Molecular Conformation
  • Molecular Structure
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Organophosphorus Compounds*
  • Thymidine / analogs & derivatives*

Substances

  • Carbon Radioisotopes
  • Indicators and Reagents
  • Oligonucleotides
  • Organophosphorus Compounds
  • 5'-O-dimethyltritylthymidine
  • Thymidine