Abstract
We describe here the conditional expression of the hepatitis B virus X protein using the inducible system controlled by a tet-responsive promoter. Induction of the X protein in Rat-2 fibroblasts activated transcription from a heterologous gene promoter and stimulated the DNA-binding activity of NFkB. The ability to produce this biologically active X protein in a stable cell line will accelerate the elucidation of the function and mechanisms of X and eventually help us understand the role of X in natural viral infection and carcinogenesis.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Animals
-
Cell Line
-
Chloramphenicol O-Acetyltransferase / metabolism
-
Fibroblasts
-
Hepatitis B virus
-
Nuclear Proteins / metabolism
-
Precipitin Tests
-
Promoter Regions, Genetic
-
Protein Synthesis Inhibitors / pharmacology
-
Rats
-
Tetracycline / pharmacology
-
Trans-Activators / biosynthesis
-
Trans-Activators / genetics*
-
Transcription, Genetic
-
Transcriptional Activation*
-
Viral Regulatory and Accessory Proteins
Substances
-
Nuclear Proteins
-
Protein Synthesis Inhibitors
-
Trans-Activators
-
Viral Regulatory and Accessory Proteins
-
hepatitis B virus X protein
-
Chloramphenicol O-Acetyltransferase
-
Tetracycline