Effect of captopril on intracellular pH in vascular smooth muscle cells

Zhongguo Yao Li Xue Bao. 1996 Sep;17(5):448-50.

Abstract

Aim: To observe the effect of captopril (Cap) on intracellular pH (pHi) in aortic smooth muscle cells (ASMC).

Methods: Cultured ASMC derived from rat and rabbit aortae were loaded with the fluorescent dye BCECF and pHi was determined using digital image processing method.

Results: The pHi of untreated SHR and WKY rats were 7.37 +/- 0.29 and 7.19 +/- 0.31, respectively. Oral Cap decreased pHi (7.11 +/- 0.26, P < 0.05) and exaggerated pHi response to angiotensin II (Ang-II, 0.1 mumol.L-1) in ASMC of SHR rats vs WKY rats (0.14 +/- 0.05 vs 0.21 +/- 0.05 pH units, P < 0.01). Cap in vitro had no effect on Ang-II induced intracellular alkalinization in ASMC of rabbits.

Conclusion: Oral Cap inhibits Na+(-)H+ exchange activity in ASMC of SHR rats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin-Converting Enzyme Inhibitors / pharmacology*
  • Animals
  • Aorta / metabolism
  • Captopril / pharmacology*
  • Cells, Cultured
  • Hydrogen-Ion Concentration
  • Male
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / drug effects*
  • Muscle, Smooth, Vascular / metabolism
  • Rabbits
  • Rats
  • Rats, Inbred SHR
  • Rats, Inbred WKY

Substances

  • Angiotensin-Converting Enzyme Inhibitors
  • Captopril