c-Cbl/Sli-1 regulates endocytic sorting and ubiquitination of the epidermal growth factor receptor

Genes Dev. 1998 Dec 1;12(23):3663-74. doi: 10.1101/gad.12.23.3663.

Abstract

Ligand-induced down-regulation of two growth factor receptors, EGF receptor (ErbB-1) and ErbB-3, correlates with differential ability to recruit c-Cbl, whose invertebrate orthologs are negative regulators of ErbB. We report that ligand-induced degradation of internalized ErbB-1, but not ErbB-3, is mediated by transient mobilization of a minor fraction of c-Cbl into ErbB-1-containing endosomes. This recruitment depends on the receptor's tyrosine kinase activity and an intact carboxy-terminal region. The alternative fate is recycling of internalized ErbBs to the cell surface. Cbl-mediated receptor sorting involves covalent attachment of ubiquitin molecules, and subsequent lysosomal and proteasomal degradation. The oncogenic viral form of Cbl inhibits down-regulation by shunting endocytosed receptors to the recycling pathway. These results reveal an endosomal sorting machinery capable of controlling the fate, and, hence, signaling potency, of growth factor receptors.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Biological Transport
  • Cell Compartmentation
  • Down-Regulation
  • Endocytosis*
  • Endosomes
  • ErbB Receptors / genetics
  • ErbB Receptors / metabolism*
  • Ligands
  • Models, Biological
  • Mutation
  • Oncogene Protein v-cbl
  • Protein Processing, Post-Translational
  • Protein-Tyrosine Kinases / metabolism
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-cbl
  • Receptor, ErbB-3
  • Retroviridae Proteins, Oncogenic / metabolism*
  • Signal Transduction
  • Ubiquitin-Protein Ligases*
  • Ubiquitins / metabolism*

Substances

  • Ligands
  • Oncogene Protein v-cbl
  • Proto-Oncogene Proteins
  • Retroviridae Proteins, Oncogenic
  • Ubiquitins
  • Proto-Oncogene Proteins c-cbl
  • Ubiquitin-Protein Ligases
  • ErbB Receptors
  • Protein-Tyrosine Kinases
  • Receptor, ErbB-3