Abstract
Purpose:
To compare conjunctival epithelium expression of HLA-DR and ICAM-1 with tear dynamics and ocular surface parameters.
Methods:
Brush cytology and flow cytometry were used to quantitatively analyze HLA-DR and ICAM-1 expression in 28 dry eye patients.
Results:
HLA-DR was expressed in 66% of the conjunctival cells of dry eye patients. This expression correlates with that of ICAM-1, as well as with the Schirmer test results (p < 0.05).
Conclusion:
This study confirmed that dry eye consists not only of ocular surface desiccation, but also of upregulation of the HLA-DR and ICAM-1 molecule in the conjunctival epithelium, possibly resulting in increased inflammation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibodies, Monoclonal
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Conjunctiva / metabolism*
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Conjunctiva / pathology
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Contrast Media
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Dry Eye Syndromes / metabolism*
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Dry Eye Syndromes / pathology
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Epithelium / metabolism
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Epithelium / pathology
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Female
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Flow Cytometry
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Fluorescein
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Fluorescent Antibody Technique
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Fluorescent Dyes
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HLA-DR Antigens / biosynthesis*
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Humans
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Intercellular Adhesion Molecule-1 / biosynthesis
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Male
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Middle Aged
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Rose Bengal
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Sjogren's Syndrome / metabolism
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Sjogren's Syndrome / pathology
Substances
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Antibodies, Monoclonal
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Contrast Media
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Fluorescent Dyes
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HLA-DR Antigens
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Intercellular Adhesion Molecule-1
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Rose Bengal
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Fluorescein